Evidence map›Paper›PMID 28129133›Full record

ArticleMolecular therapy : the journal of the American Society of Gene Therapy2017

A Targeted and Stable Polymeric Nanoformulation Enhances Systemic Delivery of mRNA to Tumors.

Qixian Chen, Ruogu Qi, Xiyi Chen, Xi Yang, Sudong Wu, Haihua Xiao, Wenfei Dong

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
3.9field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 88 citations in OpenAlex.

  1. Article
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  5. Synthetic Polymers for Drug, Gene, and Vaccine Delivery.Polymer science & technology (Washington, D.C.) · 2025
    Review
  6. Review
  7. Responsive Supramolecular Polymers for Diagnosis and Treatment.International journal of molecular sciences · 2024
    Review
  8. mRNA Delivery: Challenges and Advances through Polymeric Soft Nanoparticles.International journal of molecular sciences · 2024
    Review
  9. Delivery of mRNA with Histidine-Lysine Peptides.Methods in molecular biology (Clifton, N.J.) · 2024
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 6 institutions in 2 countries.

Qixian ChenCAS Key Laboratory of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering, Suzhou 215163, China; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. Electronic address: qixian@mit.edu.
Ruogu QiState Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
Xiyi ChenSchool of Public Health, Dalian Medical University, No. 9 West Section Lvshun South Road, Dalian 116044, China. Electronic address: xychen@dmu.edu.cn.
Xi YangDepartment of Neurosurgery, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China.
Sudong WuNingbo Institute of Material Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, China.
Haihua XiaoState Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
Wenfei DongCAS Key Laboratory of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering, Suzhou 215163, China. Electronic address: weifeidong@126.com.
Suzhou Institute of Biomedical Engineering and Technology · CNChinese Academy of Sciences · CNDalian Medical University · CNNingbo Institute of Industrial Technology · CNRenji Hospital · CNState Key Laboratory of Polymer Physics and Chemistry · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The high vulnerability of mRNA necessitates the manufacture of delivery vehicles to afford adequate protection in the biological milieu. Here, mRNA was complexed with a mixture of cRGD-poly(ethylene glycol) (PEG)-polylysine (PLys) (thiol) and poly(N-isopropylacrylamide) (PNIPAM)-PLys(thiol). The ionic complex core consisting of opposite-charged PLys and mRNA was crosslinked though redox-responsive disulfide linkage, thereby avoiding structural disassembly for exposure of mRNA to harsh biological environments. Furthermore, PNIPAM contributed to prolonged survival in systemic circulation by presenting a spatial barrier in impeding accessibility of nucleases, e.g., RNase, due to the thermo-responsive hydrophilic-hydrophobic transition behavior upon incubation at physiological temperature enabling translocation of PNIPAM from shell to intermediate barrier. Ultimately, the cRGD ligand attached to the formulation demonstrated improved tumor accumulation and potent gene expression, as manifested by virtue of facilitated cellular uptake and intracellular trafficking. These results indicate promise for the utility of mRNA as a therapeutic tool for disease treatment.

Indexed as

Drug Delivery SystemsNanoparticlesPolymersCell Line, TumorCell SurvivalDrug CompoundingHumansLigandsNeoplasmsPolyethylene GlycolsPolylysineRNA, MessengerLigandsPolyethylene GlycolsPolylysinePolymersRNA, Messengergene expressionmRNA deliverypolymeric micellethermo-responsivetumor targeting

Identifiers

PMID28129133
PMCPMC5363296
OpenAlexW2570088976

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.