ArticleNature communications2017
High-throughput automated microfluidic sample preparation for accurate microbial genomics.
Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
42 citing papers in PubMed.
- Near-real-time whole-genome sequencing in hospital infection control: a narrative review.Infection · 2026Review
- AI-driven lateral flow immunoassay for point-of-care detection of cardiac biomarkers in acute myocardial infarction.Mikrochimica acta · 2026Article
- A novel one-step multiplex PCR method (OS-MPCR) for efficient NGS library preparation in cancer.PloS one · 2026Article
- An open source platform to automate the design, verification, and manufacture of 3D printed microfluidic devices.Scientific reports · 2025Article
- De novo mutations mediate phenotypic switching in an opportunistic human lung pathogen.Nature communications · 2025Article
- Fully automated in vivo screening system for multi-organ imaging and pharmaceutical evaluation.Microsystems & nanoengineering · 2025Article
- Advancing quantitative PCR with color cycle multiplex amplification.Nucleic acids research · 2024Article
- Automation of customizable library preparation for next-generation sequencing into an open microfluidic platform.Scientific reports · 2024Article
- Utilizing cost-effective portable equipment to enhance COVID-19 variant tracking both on-site and at a large scale.Journal of clinical microbiology · 2024Article
- Rapid deep learning-assisted predictive diagnostics for point-of-care testing.Nature communications · 2024Article
- Microfluidic Capture of Mycobacterium tuberculosis from Clinical Samples for Culture-Free Whole-Genome Sequencing.Microbiology spectrum · 2023Article
- Research progress of electrode shapes in EWOD-based digital microfluidics.RSC advances · 2023Review
- Advances in point-of-care genetic testing for personalized medicine applications.Biomicrofluidics · 2023Review
- A Compact Control System to Enable Automated Operation of Microfluidic Bioanalytical Assays.Biosensors · 2022Article
- Target Enrichment Approaches for Next-Generation Sequencing Applications in Oncology.Diagnostics (Basel, Switzerland) · 2022Review
- Whole genome sequencing of cyanobacteriumiScience · 2022Article
- A droplet-based microfluidic approach to isolating functional bacteria from gut microbiota.Frontiers in cellular and infection microbiology · 2022Article
- Innovations to culturing the uncultured microbial majority.Nature reviews. Microbiology · 2021Review
- Isolation of target DNA using synergistic magnetic bead transport and electrokinetic flow.Biomicrofluidics · 2021Article
- μCB-seq: microfluidic cell barcoding and sequencing for high-resolution imaging and sequencing of single cells.Lab on a chip · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
Abstract
Low-cost shotgun DNA sequencing is transforming the microbial sciences. Sequencing instruments are so effective that sample preparation is now the key limiting factor. Here, we introduce a microfluidic sample preparation platform that integrates the key steps in cells to sequence library sample preparation for up to 96 samples and reduces DNA input requirements 100-fold while maintaining or improving data quality. The general-purpose microarchitecture we demonstrate supports workflows with arbitrary numbers of reaction and clean-up or capture steps. By reducing the sample quantity requirements, we enabled low-input (∼10,000 cells) whole-genome shotgun (WGS) sequencing of Mycobacterium tuberculosis and soil micro-colonies with superior results. We also leveraged the enhanced throughput to sequence ∼400 clinical Pseudomonas aeruginosa libraries and demonstrate excellent single-nucleotide polymorphism detection performance that explained phenotypically observed antibiotic resistance. Fully-integrated lab-on-chip sample preparation overcomes technical barriers to enable broader deployment of genomics across many basic research and translational applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.