ArticleNature communications2017
Enzyme I facilitates reverse flux from pyruvate to phosphoenolpyruvate in Escherichia coli.
Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 55 citations in OpenAlex.
- The phosphoenolpyruvate phosphotransferase system regulates cell length and width through semi-independent pathways.Molecular biology of the cell · 2026Article
- Transcriptome profiling of a novelApplied and environmental microbiology · 2026Article
- Exploring glycolytic enzymes in disease: potential biomarkers and therapeutic targets in neurodegeneration, cancer and parasitic infections.Open biology · 2025Review
- A citric acid cycle-deficient Escherichia coli as an efficient chassis for aerobic fermentations.Nature communications · 2024Article
- Engineering a SyntheticACS synthetic biology · 2024Article
- In-patient evolution of a high-persisterProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Enzyme 1 of the phosphoenolpyruvate:sugar phosphotransferase system is involved in resistance to MreB disruption in wild-type and ∆envC cells.Molecular microbiology · 2022Article
- Rewiring the microbial metabolic network for efficient utilization of mixed carbon sources.Journal of industrial microbiology & biotechnology · 2021Article
- Structure elucidation of the elusive Enzyme I monomer reveals the molecular mechanisms linking oligomerization and enzymatic activity.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- The PEP-pyruvate-oxaloacetate node: variation at the heart of metabolism.FEMS microbiology reviews · 2021Article
- Global Transcriptional Regulators Fine-Tune the Translational and Metabolic Efficiency for Optimal Growth of Escherichia coli.mSystems · 2021Article
- Biotechnological Production of the Sunscreen Pigment Scytonemin in Cyanobacteria: Progress and Strategy.Marine drugs · 2021Review
- Integrative Network Analysis Revealed Genetic Impact of Pyruvate Kinase L/R on Hepatocyte Proliferation and Graft Survival after Liver Transplantation.Oxidative medicine and cellular longevity · 2021Article
- Escherichia coli metabolism under short-term repetitive substrate dynamics: adaptation and trade-offs.Microbial cell factories · 2020Article
- High-resolutionNature protocols · 2019Article
- Unusual Phosphoenolpyruvate (PEP) Synthetase-Like Protein Crucial to Enhancement of Polyhydroxyalkanoate Accumulation in Haloferax mediterranei Revealed by Dissection of PEP-Pyruvate Interconversion Mechanism.Applied and environmental microbiology · 2019Article
- Reprogramming of gene expression in Escherichia coli cultured on pyruvate versus glucose.Molecular genetics and genomics : MGG · 2019Article
- From Escherichia coli mutant 13C labeling data to a core kinetic model: A kinetic model parameterization pipeline.PLoS computational biology · 2019Article
- Dissecting the genetic and metabolic mechanisms of adaptation to the knockout of a major metabolic enzyme inProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Fast growth phenotype of E. coli K-12 from adaptive laboratory evolution does not require intracellular flux rewiring.Metabolic engineering · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The bacterial phosphoenolpyruvate-carbohydrate phosphotransferase system (PTS) consists of cascading phosphotransferases that couple the simultaneous import and phosphorylation of a variety of sugars to the glycolytic conversion of phosphoenolpyruvate (PEP) to pyruvate. As the primary route of glucose uptake in E. coli, the PTS plays a key role in regulating central carbon metabolism and carbon catabolite repression, and is a frequent target of metabolic engineering interventions. Here we show that Enzyme I, the terminal phosphotransferase responsible for the conversion of PEP to pyruvate, is responsible for a significant in vivo flux in the reverse direction (pyruvate to PEP) during both gluconeogenic and glycolytic growth. We use
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