ArticleIn vitro cellular & developmental biology. Animal2017
Effect of sodium butyrate on cell proliferation and cell cycle in porcine intestinal epithelial (IPEC-J2) cells.
Article in In vitro cellular & developmental biology. Animal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 36 citations in OpenAlex.
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- Optimum blue light exposure: a means to increase cell-specific productivity in Chinese hamster ovary cells.Applied microbiology and biotechnology · 2024Article
- Cell migration and proliferation capacity of IPEC-J2 cells after short-chain fatty acid exposure.PloS one · 2024Article
- Molecular mechanism of valine and its metabolite in improving triglyceride synthesis of porcine intestinal epithelial cells.Scientific reports · 2023Article
- Transcriptomic analysis reveals mode of action of butyric acid supplementation in an intensified CHO cell fed-batch process.Biotechnology and bioengineering · 2022Article
- Effects of different short-chain fatty acids (SCFA) on gene expression of proteins involved in barrier function in IPEC-J2.Porcine health management · 2022Article
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- MicroRNA-367 directly targets PIK3R3 to inhibit proliferation and invasion of oral carcinoma cells.Bioscience reports · 2020Article
- MicroRNA-96-5p promotes proliferation, invasion and EMT of oral carcinoma cells by directly targeting FOXF2.Biology open · 2020Article
- MiR-338 regulates NFATc1 expression and inhibits the proliferation and epithelial-mesenchymal transition of human non-small-cell lung cancer cells.Molecular genetics & genomic medicine · 2020Article
- Dynamic Changes in Serum Metabolomic Profiles of Growing Pigs Induced by Intravenous Infusion of Sodium Butyrate.Metabolites · 2020Article
- miR-216a-5p promotes mesangial cell proliferation by targeting FoxO1 in diabetic nephropathy.International journal of clinical and experimental pathology · 2019Article
- Effects of Quercetin on Proliferation and H₂O₂-Induced Apoptosis of Intestinal Porcine Enterocyte Cells.Molecules (Basel, Switzerland) · 2018Article
- Lactobacillus plantarum Enhanced IL-22 Production in Natural Killer (NK) Cells That Protect the Integrity of Intestinal Epithelial Cell Barrier Damaged by Enterotoxigenic Escherichia coli.International journal of molecular sciences · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Conflicting results have been reported that butyrate in normal piglets leads either to an increase or to a decrease of jejunal villus length, implying a possible effect on the proliferation of enterocytes. No definitive study was found for the biological effects of butyrate in porcine jejunal epithelial cells. The present study used IPEC-J2 cells, a non-transformed jejunal epithelial line to evaluate the direct effects of sodium butyrate on cell proliferation, cell cycle regulation, and apoptosis. Low concentrations (0.5 and 1 mM) of butyrate had no effect on cell proliferation. However, at 5 and 10 mM, sodium butyrate significantly decreased cell viability, accompanied by reduced levels of p-mTOR and PCNA protein. Sodium butyrate, in a dose-dependent manner, induced cell cycle arrest in G0/G1 phase and reduced the numbers of cells in S phase. In addition, relative expression of p21, p27, and pro-apoptosis bak genes, and protein levels of p21Waf1/Cip1, p27Kip1, cyclinD3, CDK4, and Cleave-caspase3 were increased by higher concentrations of sodium butyrate (1, 5, 10 mM), and the levels of cyclinD1 and CDK6 were reduced by 5 and 10 mM butyrate. Butyrate increased the phosphorylated form of the signaling molecule p38 and phosphorylated JNK. In conclusion, the present in vitro study indicated that sodium butyrate inhibited the proliferation of IPEC-J2 cells by inducing cell cycle arrest in the G0/G1 phase of cell cycles and by increasing apoptosis at high concentrations.
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