ArticleAnalytical methods : advancing methods and applications2016
Multiplexed detection of pathogen-specific DNA using engineered zinc finger proteins without target amplification.
Article in Analytical methods : advancing methods and applications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
4 citing papers in PubMed.
- Advances in Double-Stranded DNA Targeting Technologies.Exploration (Beijing, China) · 2026Review
- Application of Engineered Zinc Finger Proteins Immobilized on Paramagnetic Beads for Multiplexed Detection of Pathogenic DNA.Journal of microbiology and biotechnology · 2021Article
- Ultrasensitive cloth-based microfluidic chemiluminescence detection of Listeria monocytogenes hlyA gene by hemin/G-quadruplex DNAzyme and hybridization chain reaction signal amplification.Analytical and bioanalytical chemistry · 2020Article
- Pathogen-specific DNA sensing with engineered zinc finger proteins immobilized on a polymer chip.The Analyst · 2018Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Multiplexed detection of pathogen-specific DNA sequences in a simple and reliable way is in great demand for clinical and biomedical applications. However, there is still a lack of available DNA detection methods that are simple and pathogen-selective for point-of-care (POC) testing. Here, we report a novel zinc finger protein (ZFP)-based chemiluminescent method for direct detection of pathogenic double-stranded DNA (dsDNA) in a multiplexed platform. ZFPs are custom-designed to identify unique pathogenic DNA sequences. ZFP-based chemiluminescent detection of dsDNA provides sufficient sensitivity (≤50 fmol) and high specificity without target DNA amplification. Our study addresses the potential of developing a simple and selective pathogen detection method in a multiplexed fashion needed for POC application.
Identifiers
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Registered trials
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