ArticlePloS one2017
Usefulness of MALDI-TOF MS as a Diagnostic Tool for the Identification of Streptococcus Species Recovered from Clinical Specimens of Pigs.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed.
- Silver Nanoparticles in Donkey Semen Stored at 17°C: Effect on Sperm Parameters and Bacterial Growth.Reproduction in domestic animals = Zuchthygiene · 2026Article
- Cinnamaldehyde as a Natural Antimicrobial in Donkey Semen: Efficacy and Sperm Cytotoxicity.Reproduction in domestic animals = Zuchthygiene · 2026Article
- Antimicrobial Resistance and Genomic Characterization of a Novel ST181Antibiotics (Basel, Switzerland) · 2026Article
- Meningitis in a French pig farmer caused by a serotype 2ASM case reports · 2026Article
- Evaluation of the antimicrobial susceptibility and resistance gene mapping ofOpen veterinary journal · 2026Article
- Genomic re-evaluation of clinical isolates reveals a structuredJournal of clinical microbiology · 2025Article
- Disease-associated Streptococcus suis (DASS) in lactation: detection patterns and implications for control.Porcine health management · 2025Article
- Pathological and bacteriological findings in sows, finisher pigs, and piglets, being culled for lameness.Porcine health management · 2025Article
- Characterization of neurologic disease-associatedJournal of clinical microbiology · 2024Article
- Whole-Genome Sequencing Evaluation of MALDI-TOF MS as a Species Identification Tool for Streptococcus suis.Journal of clinical microbiology · 2021Article
- Rapid Detection ofFoods (Basel, Switzerland) · 2021Article
- Streptococcus suis serotyping by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.PloS one · 2021Article
- Molecular subtyping and antimicrobial susceptibility ofJournal of veterinary science · 2020Article
- Isolation and characteristics of multi-drug resistant Streptococcus porcinus from the vaginal secretions of sow with endometritis.BMC veterinary research · 2020Article
- Identification of bacterial pathogens in cultured fish with a custom peptide database constructed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).BMC veterinary research · 2020Article
- Serotype and Genotype (Multilocus Sequence Type) of Streptococcus suis Isolates from the United States Serve as Predictors of Pathotype.Journal of clinical microbiology · 2019Article
- High rate misidentification of biochemically determined Streptococcus isolates from swine clinical specimens.The Journal of veterinary medical science · 2019Article
- Molecular Cloning and Docking ofFrontiers in microbiology · 2018Article
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6 authors.
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Abstract
The application of MALDI-TOF MS for identifying streptococcal isolates recovered from clinical specimens of diseased pigs was evaluated. For this proposal, the MALDI BDAL Database (Bruker Daltoniks, Germany) was supplemented with the main spectrum profiles (MSP) of the reference strains of S. porci, S. porcorum and S. plurextorum associated with pneumonia and septicemia. Although these three species showed similar MALDI profiles, several peaks were recognized that can be useful for their differentiation: S. porci (4113, 6133, 7975 and 8228 m/z Da), S. plurextorum (3979, 4078, 4665, 6164, 6491, 6812, 7959 and 9330 m/z Da) and S. porcorum (3385, 3954, 4190, 6772, 7908, and 8381 m/z Da). After adding these MSPs, an evaluation was conducted to determine the accuracy of MALDI-TOF MS for the identification of streptococci from diseased pigs using 74 field isolates. Isolates were identified as S. suis, S. porcinus, S. dysgalactiae, S. hyovaginalis, S. porcorum, S. alactolyticus, S. hyointestinalis and S. orisratti. This is the first time that the latter three species have been reported from clinical specimens of pigs. Overall, there was good concordance (95.9%) between the results obtained from MALDI-TOF MS identification (best hint) and those from genotyping. Our results demonstrate the good performance of MALDI-TOF MS (100% sensitivity and specificity) for identifying most of the species of streptococci that can frequently be isolated from diseased pigs. However, conflicting results were observed in the correct identification of some isolates of S. dysgalactiae and S. alactolyticus.
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