ArticlePloS one2017
Differences in AMY1 Gene Copy Numbers Derived from Blood, Buccal Cells and Saliva Using Quantitative and Droplet Digital PCR Methods: Flagging the Pitfall.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT01804738 (Investigating Inter-individual Variability in Glycemic and Insulin Responses), which is not on this map. Cited by 10 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Investigating Inter-individual Variability in Glycemic and Insulin Responses
Who cites it
10 citing papers in PubMed.
- Body Mass Index and Inter-Individual Variation in Salivary Amylase Gene Copy Number Assessed Using an In-House Validated Method: A Pilot Study from the National Nutrition Survey of a High Rice-Consuming Nation.Lifestyle genomics · 2026Article
- Effects of salivary amylase gene copy number on metabolic health.Frontiers in nutrition · 2026Review
- Obesity Parameters in Women Is Associated With AMY1 Gene Copy Number, Nesfatin-1 Level, and Dietary Intake: A Case-Control Study.Molecular nutrition & food research · 2025Article
- The association between salivary amylase gene copy number and enzyme activity with type 2 diabetes status.PloS one · 2025Article
- The Effectiveness of ddPCR for Detection of Point Mutations in Poor-Quality Saliva Samples.Healthcare (Basel, Switzerland) · 2022Article
- Effect of AMY1 copy number variation and various doses of starch intake on glucose homeostasis: data from a cross-sectional observational study and a crossover meal study.Genes & nutrition · 2021Article
- Development and Evaluation of a Droplet Digital PCR Assay for 8p23 β-Defensin Cluster Copy Number Determination.Molecular diagnosis & therapy · 2021Article
- High plasma salivary α-amylase, but not high AMY1 copy number, associated with low obesity rate in Qatari adults: cross-sectional study.Scientific reports · 2020Article
- Copy Number Variation of the Salivary Amylase Gene and Glucose Metabolism in Healthy Young Japanese Women.Journal of clinical medicine research · 2020Article
- Increased Inflammation and Cardiometabolic Risk in Individuals with LowJournal of clinical medicine · 2019Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionThe human salivary (AMY1) gene, encoding salivary α-amylase, has variable copy number variants (CNVs) in the human genome. We aimed to determine if real-time quantitative polymerase chain reaction (qPCR) and the more recently available Droplet Digital PCR (ddPCR) can provide a precise quantification of the AMY1 gene copy number in blood, buccal cells and saliva samples derived from the same individual.
methodsSeven participants were recruited and DNA was extracted from the blood, buccal cells and saliva samples provided by each participant. Taqman assay real-time qPCR and ddPCR were conducted to quantify AMY1 gene copy numbers. Statistical analysis was carried out to determine the difference in AMY1 gene copy number between the different biological specimens and different assay methods.
resultsWe found significant within-individual difference (p<0.01) in AMY1 gene copy number between different biological samples as determined by qPCR. However, there was no significant within-individual difference in AMY1 gene copy number between different biological samples as determined by ddPCR. We also found that AMY1 gene copy number of blood samples were comparable between qPCR and ddPCR, while there is a significant difference (p<0.01) between AMY1 gene copy numbers measured by qPCR and ddPCR for both buccal swab and saliva samples.
conclusionsDespite buccal cells and saliva samples being possible sources of DNA, it is pertinent that ddPCR or a single biological sample, preferably blood sample, be used for determining highly polymorphic gene copy numbers like AMY1, due to the large within-individual variability between different biological samples if real time qPCR is employed.
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