ArticlePloS one2017
Profiling Living Bacteria Informs Preparation of Fecal Microbiota Transplantations.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
50 citing papers in PubMed, 1 synthesis or guideline pooled it, 91 citations in OpenAlex.
- Impact of Fecal Microbiota Transplantation on Obesity and Metabolic Syndrome-A Systematic Review.Nutrients · 2019Pooled it
- Trial
- Trial
- Method for assessing inter-individual variability in the carbohydrate utilization potential of gut microbiota ex vivo.MethodsX · 2026Article
- Fecal microbiota from chronically stressed donors transfers behavioral and prefrontal cortex gene expression alterations to healthy recipient mice.Neurobiology of stress · 2026Article
- Viable gut bacterial metrics associated with intestinal eubiosis and dysbiosis.Gut microbes reports · 2026Article
- Absolute quantification of the living skin microbiome overcomes relic-DNA bias and reveals specific patterns across volunteers.Microbiome · 2025Article
- Reconstruction characteristics of gut microbiota from patients with type 1 diabetes affect the phenotypic reproducibility of glucose metabolism in mice.Science China. Life sciences · 2025Article
- Trehalose and maltodextrin preserve microbial community structure in freeze-dried fecal samples for fecal microbiota transplantation.ISME communications · 2025Article
- Laboratory preparation methods for human-derived fecal microbial suspensions for fecal microbiota transplantation: a review and standardization perspectives.Frontiers in microbiology · 2025Review
- Fecal Microbiota Transplantation: Indications, Methods, and Challenges.Journal of microbiology (Seoul, Korea) · 2024Review
- Evaluating Bacterial Viability in Faecal Microbiota Transplantation: A Comparative Analysis of In Vitro Cultivation and Membrane Integrity Methods.Journal of clinical laboratory analysis · 2024Article
- Multi-Omics Analysis Unravels the Impact of Stool Sample Logistics on Metabolites and Microbial Composition.Microorganisms · 2024Article
- The practice of fecal microbiota transplantation in inflammatory bowel disease.Intestinal research · 2024Review
- Anaerobic Feces Processing for Fecal Microbiota Transplantation Improves Viability of Obligate Anaerobes.Microorganisms · 2023Article
- Fecal microbiota transplantation in patients with metabolic syndrome and obesity: A randomized controlled trial.World journal of clinical cases · 2023Article
- Accelerating Gut Microbiome Research with Robust Sample Collection.Research & reviews. Journal of microbiology and biotechnology · 2023Article
- Evaluation of Bacterial Composition and Viability of Equine Feces after Processing for Transplantation.Microorganisms · 2023Article
- Long-term ecological and evolutionary dynamics in the gut microbiomes of carbapenemase-producing Enterobacteriaceae colonized subjects.Nature microbiology · 2022Article
- Microbiota Transplantation in an Antibiotic-Induced Bacterial Depletion Mouse Model: Reproducible Establishment, Analysis, and Application.Microorganisms · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fecal microbiota transplantation is a compelling treatment for recurrent Clostridium difficile infections, with potential applications against other diseases associated with changes in gut microbiota. But variability in fecal bacterial communities-believed to be the therapeutic agent-can complicate or undermine treatment efficacy. To understand the effects of transplant preparation methods on living fecal microbial communities, we applied a DNA-sequencing method (PMA-seq) that uses propidium monoazide (PMA) to differentiate between living and dead fecal microbes, and we created an analysis pipeline to identify individual bacteria that change in abundance between samples. We found that oxygen exposure degraded fecal bacterial communities, whereas freeze-thaw cycles and lag time between donor defecation and transplant preparation had much smaller effects. Notably, the abundance of Faecalibacterium prausnitzii-an anti-inflammatory commensal bacterium whose absence is linked to inflammatory bowel disease-decreased with oxygen exposure. Our results indicate that some current practices for preparing microbiota transplant material adversely affect living fecal microbial content and highlight PMA-seq as a valuable tool to inform best practices and evaluate the suitability of clinical fecal material.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.