ArticleJournal of the American Chemical Society2017
Dynamics of a DNA Mismatch Site Held in Confinement Discriminate Epigenetic Modifications of Cytosine.
Article in Journal of the American Chemical Society, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Aldehyde or Hydrate? Investigation into the Oxidation of 5-Formylcytosine Derivatives Using a Computational and Experimental Approach.Chembiochem : a European journal of chemical biology · 2025Article
- Bisulfite and Nanopore Sequencing for Pseudouridine in RNA.Accounts of chemical research · 2023Article
- Biological nanopores for single-molecule sensing.iScience · 2022Review
- The pH-Dependence of the Hydration of 5-Formylcytosine: an Experimental and Theoretical Study.Chembiochem : a European journal of chemical biology · 2022Article
- Stochasticity in Single-Entity Electrochemistry.Current opinion in electrochemistry · 2021Article
- Detection and Discrimination of DNA Adducts Differing in Size, Regiochemistry, and Functional Group by Nanopore Sequencing.Chemical research in toxicology · 2020Article
- Impact of 5-formylcytosine on the melting kinetics of DNA by 1H NMR chemical exchange.Nucleic acids research · 2020Article
- Dynamic interaction of fluoroquinolones with magnesium ions monitored using bacterial outer membrane nanopores.Chemical science · 2020Article
- DNA nanotechnology assisted nanopore-based analysis.Nucleic acids research · 2020Review
- Microfluidic epigenomic mapping technologies for precision medicine.Lab on a chip · 2019Review
- Biomimetic Membranes with Transmembrane Proteins: State-of-the-Art in Transmembrane Protein Applications.International journal of molecular sciences · 2019Review
- Displacement chemistry-based nanopore analysis of nucleic acids in complicated matrices.Chemical communications (Cambridge, England) · 2018Article
- Highly Selective 5-Formyluracil Labeling and Genome-wide Mapping Using (2-Benzimidazolyl)Acetonitrile Probe.iScience · 2018Article
- γ-Hemolysin Nanopore Is Sensitive to Guanine-to-Inosine Substitutions in Double-Stranded DNA at the Single-Molecule Level.Journal of the American Chemical Society · 2018Article
- Nanoscale electrochemical kinetics & dynamics: the challenges and opportunities of single-entity measurements.Faraday discussions · 2018Article
- Single-Molecule Titration in a Protein Nanoreactor Reveals the Protonation/Deprotonation Mechanism of a C:C Mismatch in DNA.Journal of the American Chemical Society · 2018Article
- Nanopore Analysis of the 5-Guanidinohydantoin to Iminoallantoin Isomerization in Duplex DNA.The Journal of organic chemistry · 2018Article
- Membrane protein-based biosensors.Journal of the Royal Society, Interface · 2018Review
- Sequence-Specific Covalent Capture Coupled with High-Contrast Nanopore Detection of a Disease-Derived Nucleic Acid Sequence.Chembiochem : a European journal of chemical biology · 2017Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The identification and discrimination of four epigenetic modifications to cytosine in the proposed active demethylation cycle is demonstrated at the single-molecule level, without the need for chemical pretreatment or labeling. The wild-type protein nanopore α-hemolysin is used to capture individual DNA duplexes containing a single cytosine-cytosine mismatch. The mismatch is held at the latch constriction of α-hemolysin, which is used to monitor the kinetics of base-flipping at the mismatch site. Base-flipping and the subsequent interactions between the DNA and the protein are dramatically altered when one of the cytosine bases is replaced with methyl-, hydroxymethyl-, formyl-, or carboxylcytosine. As well as providing a route to single-molecule analysis of important epigenetic markers in DNA, our results provide important insights into how the introduction of biologically relevant, but poorly understood, modifications to cytosine affect the local conformational dynamics of a DNA duplex in a confined environment.
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