Evidence map›Paper›PMID 28124257›Full record

ArticleInflammation2017

3'UTR AU-Rich Elements (AREs) and the RNA-Binding Protein Tristetraprolin (TTP) Are Not Required for the LPS-Mediated Destabilization of Phospholipase-Cβ-2 mRNA in Murine Macrophages.

Smita Shukla, Genie Elson, Perry J Blackshear, Carol S Lutz, S Joseph Leibovich

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Inflammation, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.7field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 12 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Smita ShuklaDepartment of Cell Biology & Molecular Medicine, New Jersey Medical School, Rutgers University School of Biomedical and Health Sciences, 185 South Orange Avenue, Newark, NJ, 07103, USA.
Genie ElsonDepartment of Cell Biology & Molecular Medicine, New Jersey Medical School, Rutgers University School of Biomedical and Health Sciences, 185 South Orange Avenue, Newark, NJ, 07103, USA.
Perry J BlackshearThe Post-Transcriptional Gene Expression Group, National Institute of Environmental Health Sciences, Research Triangle Park, Durham, NC, 27709, USA.
Carol S LutzDepartment of Microbiology, Biochemistry and Molecular Genetics, New Jersey Medical School, Rutgers University School of Biomedical and Health Sciences, 185 South Orange Avenue, Newark, NJ, 07103, USA.
S Joseph LeibovichDepartment of Cell Biology & Molecular Medicine, New Jersey Medical School, Rutgers University School of Biomedical and Health Sciences, 185 South Orange Avenue, Newark, NJ, 07103, USA. leibovic@njms.rutgers.edu.
Rutgers, The State University of New Jersey · USNational Institute of Environmental Health Sciences · US

Funding

TTP and related proteins in the regulation of gene expressionZIAES090080 · NIEHS · NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES · PI BLACKSHEAR, PERRY J · 2009 to 2025
$52.6M
TTP AND RELATED PROTEINS IN INFLAMMATORY DISEASESZ01ES090080 · NIEHS · NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES · PI BLACKSHEAR, PERRY J · 1998 to 2008
$5.3M
A role for miRNAs in adenosine-dependent alternative macrophage activationR21AI097731 · NIAID · UNIV OF MED/DENT OF NJ-NJ MEDICAL SCHOOL · PI LEIBOVICH, SAMUEL JOSEPH · 2012 to 2013
$422k
Intramural NIH HHS Z01 ES090080NIAID NIH HHS R21 AI097731
6 · The paper itself

Abstract

We have shown previously that bacterial lipopolysaccharide (LPS)-mediated suppression of phospholipase-Cβ-2 (PLCβ-2) expression is involved in M1 (inflammatory) to M2-like (wound healing) phenotypic switching of macrophages triggered by adenosine. This suppression is mediated post-transcriptionally by destabilization of PLCβ-2 mRNA (messenger ribonucleic acid). To investigate the mechanism of this LPS-mediated destabilization, we examined the roles of RNA-binding agents including microRNAs and RNA-binding proteins that are involved in regulating stability of mRNAs encoding growth factors, inflammatory mediators, and proto-oncogenes. Adenylate and uridylate (AU)-rich elements (AREs) in 3'UTRs are specific recognition sites for RNA-binding proteins including tristetraprolin (TTP), HuR, and AUF1 and for microRNAs that are involved in regulating mRNA stability. In this study, we investigated the role of TTP and AREs in regulating PLCβ-2 mRNA stability. The 3'UTR of the PLCβ-2 gene was inserted into the pLightswitch luciferase reporter plasmid and transfected into RAW264.7 cells. LPS suppressed luciferase expression from this reporter. Luciferase expression from mutant 3'UTR constructs lacking AREs was similarly downregulated, suggesting that these regions are not required for LPS-mediated suppression of PLCβ-2. TTP was rapidly upregulated in both primary murine macrophages and RAW264.7 cells in response to LPS. Suppression of PLCβ-2 by LPS was examined using macrophages from mice lacking TTP (TTP

Indexed as

3' Untranslated RegionsAnimalsAU Rich ElementsCells, CulturedDiabetes Mellitus, ExperimentalLipopolysaccharidesMacrophagesMicePhospholipase C betaRAW 264.7 CellsRNA-Binding ProteinsRNA StabilityTristetraprolin3' Untranslated RegionsLipopolysaccharidesPhospholipase C betaPlcb2 protein, mouseRNA-Binding ProteinsTristetraprolin3’UTRAU-rich elementslipopolysaccharide (LPS)macrophagesphospholipase-Cβ-2tristetraprolin

Identifiers

PMID28124257
PMCPMC5592789
OpenAlexW2581787600

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.