Evidence map›Paper›PMID 28124254›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2017

Probing Activation and Deactivation of the BRASSINOSTEROID INSENSITIVE1 Receptor Kinase by Immunoprecipitation.

Sara Martins, Grégory Vert, Yvon Jaillais

Open access · greenAbstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 99% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 3 institutions in 1 country.

Sara MartinsInstitute for Integrative Biology of the Cell (I2BC), Centre National de la Recherche Scientifique, Commissariat à l'Energie Atomique, Université Paris Sud, Université Paris-Saclay, 91198, Gif-sur-Yvette, France.
Grégory VertInstitute for Integrative Biology of the Cell (I2BC), Centre National de la Recherche Scientifique, Commissariat à l'Energie Atomique, Université Paris Sud, Université Paris-Saclay, 91198, Gif-sur-Yvette, France.
Yvon JaillaisLaboratoire de Reproduction et Développement des Plantes, Université de Lyon, Ecole Nationale Supérieure de Lyon, Université ClaudeBernard Lyon 1,, Université de Lyon, Ecole Nationale Supérieure de Lyon, Université Claude Bernard Lyon 1, Centre National de la Recherche Scientifique, Institut National de la Recheche Agronomique, 69342, Lyon, France. yvon.jaillais@ens-lyon.fr.
Commissariat à l'Énergie Atomique et aux Énergies Alternatives · FRLaboratoire Reproduction et Développement des Plantes · FRUniversité Paris-Sud · FR

Funding

European Research Council 336360
6 · The paper itself

Abstract

Brassinosteroids (BRs) are sterol-derived hormones that control plant growth and development. The BR receptor complex is encoded by the BRASSINOSTEROID INSENSITIVE1 (BRI1) and members of the SOMATIC EMBRYOGENESIS RECEPTOR KINASE family. For activation and deactivation, the BR receptor complex uses different posttranslational modifications and recruits various partner proteins. Here, we describe optimized immunoprecipitation protocols and variants for biochemical analyses of posttranslational modifications of BRI1 and of protein-protein interactions.

Indexed as

Gene Expression Regulation, PlantProtein Processing, Post-TranslationalArabidopsisArabidopsis ProteinsBlotting, WesternBrassinosteroidsEnzyme ActivationImmunoprecipitationPhosphorylationPlants, Genetically ModifiedProtein BindingProtein KinasesSeedlingsSignal TransductionUbiquitinationArabidopsis ProteinsBrassinosteroidsBRI1 protein, ArabidopsisProtein KinasesSERK1 protein, ArabidopsisBrassinosteroidsBRI1ImmunoprecipitationPhosphorylationProtein–protein interactionUbiquitination

Identifiers

PMID28124254
PMCPMC5321509
OpenAlexW2582725767

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.