Evidence map›Paper›PMID 28124136›Full record

ArticleParasitology research2017

New techniques to collect live Sarcoptes scabiei and evaluation of methods as alternative diagnostics for infection.

Nobuhide Kido, Teruo Akuta, Hiroshi Tarui, Keitaro Imaizumi, Takafumi Ueda, Yasuo Ono, Takane Kikuchi-Ueda, Sohei Tanaka, Tomoko Omiya

Abstract read
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In one paragraph

Article in Parasitology research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed, 1 pooled it
0.6field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 1 synthesis or guideline pooled it, 8 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Nobuhide KidoKanazawa Zoological Gardens, 5-15-1 Kamariyahigashi, Kanazawa-ku, Yokohama, 236-0042, Japan. kido@hama-midorinokyokai.or.jp.
Teruo AkutaDepartment of Microbiology and Immunology, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi-ku, Tokyo, 173-8605, Japan.
Hiroshi TaruiDivision of Genomic Technologies, Center for Life Science Technologies, RIKEN Yokohama Institute, 1-7-22 Suehiro-cho, Yokohama, 230-0045, Japan.
Keitaro ImaizumiDepartment of Microbiology and Immunology, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi-ku, Tokyo, 173-8605, Japan.
Takafumi UedaDepartment of Microbiology and Immunology, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi-ku, Tokyo, 173-8605, Japan.
Yasuo OnoDepartment of Microbiology and Immunology, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi-ku, Tokyo, 173-8605, Japan.
Takane Kikuchi-UedaDepartment of Microbiology and Immunology, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi-ku, Tokyo, 173-8605, Japan.
Sohei TanakaKanazawa Zoological Gardens, 5-15-1 Kamariyahigashi, Kanazawa-ku, Yokohama, 236-0042, Japan.
Tomoko OmiyaKanazawa Zoological Gardens, 5-15-1 Kamariyahigashi, Kanazawa-ku, Yokohama, 236-0042, Japan.
Teikyo University · JPKanazawa Hospital · JPNihon Nohyaku (Japan) · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Sarcoptes scabiei is a widespread, highly contagious skin disease that affects many mammals including humans. The biological characteristics of S. scabiei remain unclear. Therefore, the ability to collect adequate amount of mites for studies is required to advance our understanding of the parasite. The present study aimed to find a method to collect an adequate amount of live S. scabiei mites within a short time frame. The cornified layer and fur from an infected raccoon dog were inserted into a 50-ml catheter tip-type syringe. A 1.5-ml microtube was attached at the tip of the syringe to collect the mites, which crawled out from the cornified layer and fur. Four conditions were examined, and the following condition was determined to be the best: the syringe and microtube were shaded by aluminum foil, and the microtube was heated using a pet heater (36 °C). In addition, the effectiveness of this method as an alternative method to diagnose S. scabiei infections in animal was evaluated. S. scabiei live mites were not detected in the raccoon dog samples 24 h after the administration of medication (ivermectin or selamectin). The present study revealed that this technique was useful to collect adequate amounts of live mites, and the mites prefer a heated environment and actively move when using the shaded conditions. In addition, this technique was effective as an alternative diagnostic technique to detect live mites on an animal body.

Indexed as

Disease Models, AnimalAnimalsDogsHumansIvermectinMammalsRaccoon DogsSarcoptes scabieiScabiesSkinIvermectinselamectinAlternative diagnostic techniqueCharacteristics of the Sarcoptes scabiei mitesMite extractionRaccoon dogScabicidal agent

Identifiers

PMID28124136
OpenAlexW2582876873

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.