ArticleBiophysical journal2017
Mechanical Characterization of Microengineered Epithelial Cysts by Using Atomic Force Microscopy.
Article in Biophysical journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 28 citations in OpenAlex.
- Biophysics of lumen morphogenesis.Development (Cambridge, England) · 2026Review
- Molecular Motors Orchestrate Pause-and-Run Dynamics to Facilitate Intracellular Transport.Biomolecules · 2026Article
- Molecular motors orchestrate pause-and-run dynamics to facilitate intracellular transport.bioRxiv : the preprint server for biology · 2025Article
- Emergent mechanics of a networked multivalent protein condensate.Nature communications · 2025Article
- Microtubule-associated protein MAP7 promotes tubulin posttranslational modifications and cargo transport to enable osmotic adaptation.Developmental cell · 2024Article
- Assessment of Tilapia Skin Collagen for Biomedical Research Applications in Comparison with Mammalian Collagen.Molecules (Basel, Switzerland) · 2024Article
- Macromolecular Crowding Tailors the Microtubule Cytoskeleton Through Tubulin Modifications and Microtubule-Associated Proteins.bioRxiv : the preprint server for biology · 2023Article
- Material properties of phase-separated TFEB condensates regulate the autophagy-lysosome pathway.The Journal of cell biology · 2022Article
- Edible Bird's Nest, an Asian Health Food Supplement, Possesses Moisturizing Effect by Regulating Expression of Filaggrin in Skin Keratinocyte.Frontiers in pharmacology · 2021Article
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Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most organs contain interconnected tubular tissues that are one-cell-thick, polarized epithelial monolayers enclosing a fluid-filled lumen. Such tissue organization plays crucial roles in developmental and normal physiology, and the proper functioning of these tissues depends on their regulation by complex biochemical perturbations and equally important, but poorly understood, mechanical perturbations. In this study, by combining micropatterning techniques and atomic force microscopy, we developed a simple in vitro experimental platform for characterizing the mechanical properties of the MDCK II cyst, the simplest model of lumen-enclosing epithelial monolayers. By using this platform, we estimated the elasticity of the cyst monolayer and showed that the presence of a luminal space influences cyst mechanics substantially, which could be attributed to polarization and tissue-level coordination. More interestingly, the results from force-relaxation experiments showed that the cysts also displayed tissue-level poroelastic characteristics that differed slightly from those of single cells. Our study provides the first quantitative findings, to our knowledge, on the tissue-level mechanics of well-polarized epithelial cysts and offers new insights into the interplay between cyst mechanics and cyst physiology. Moreover, our simple platform is a potentially useful tool for enhancing the current understanding of cyst mechanics in health and disease.
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