Evidence map›Paper›PMID 28122222›Full record

ArticleBiophysical journal2017

Charge, Diffusion, and Current Fluctuations of Single-Stranded DNA Trapped in an MspA Nanopore.

Stephen J Fleming, Bo Lu, Jene A Golovchenko

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biophysical journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Salt-Mediated Nanopore Detection of ADAM-17.ACS applied bio materials · 2019
    Article
  4. Rapid Nanopore Assay for Carbapenem-ResistantFrontiers in microbiology · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Stephen J FlemingDepartment of Physics, Harvard University, Cambridge, Massachusetts.
Bo LuDepartment of Physics, Harvard University, Cambridge, Massachusetts.
Jene A GolovchenkoDepartment of Physics, Harvard University, Cambridge, Massachusetts; School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts. Electronic address: golovchenko@physics.harvard.edu.

Funding

Electronic Sequencing in NanoporesR01HG003703 · NHGRI · HARVARD UNIVERSITY · PI BRANTON, DANIEL NONE, GOLOVCHENKO, JENE A · 2005 to 2014
$16.1M
NHGRI NIH HHS R01 HG003703
6 · The paper itself

Abstract

We report effective charges and diffusion constants of several different single-stranded DNA oligonucleotides trapped in an MspA nanopore. Nucleotide identity is found to have a substantial influence on effective charges and diffusion constants. These quantities are determined from escape time experiments for a DNA molecule attached to a NeutrAvidin molecule that, unlike the DNA, does not pass through the pore. Correlations are reported between oligonucleotide effective charges and current blockages, and between their diffusion constants and DNA-induced current blockage fluctuations. We also report an unanticipated source of current fluctuations that reflects a discrete blockage current level structure. We posit that this is associated with interactions between the NeutrAvidin molecule and the MspA nanopore.

Indexed as

Electric ConductivityNanoporesDiffusionDNA, Single-StrandedPorinsDNA, Single-StrandedmspA protein, Mycobacterium smegmatisPorins

Identifiers

PMID28122222
PMCPMC5266147

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.