ArticleeLife2017
A general method for determining secondary active transporter substrate stoichiometry.
Article in eLife, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 38 citations in OpenAlex.
- Cooperative mechanism of neurotransmitter recognition and transport by the human vesicular polyamine transporter.Nature communications · 2026Article
- Functional role of a structural water in the elevator domain of dicarboxylate transporter VcINDY.PNAS nexus · 2026Article
- Multimodal dynamics control activity of a glial glutamate transporter.bioRxiv : the preprint server for biology · 2026Article
- Elevator mechanism dynamics in a sodium-coupled dicarboxylate transporter.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Transport of metformin metabolites by guanidinium exporters of the small multidrug resistance family.The Journal of general physiology · 2024Article
- Vesicular glutamate transporters are HNature communications · 2023Article
- Structural basis of ion - substrate coupling in the NaNature communications · 2022Article
- Assembly and Functional Role of PACE Transporter PA2880 from Pseudomonas aeruginosa.Microbiology spectrum · 2022Article
- General principles of secondary active transporter function.Biophysics reviews · 2022Review
- Elucidating the Mechanism Behind Sodium-Coupled Neurotransmitter Transporters by Reconstitution.Neurochemical research · 2022Review
- On the Role of a Conserved Methionine in the NaNeurochemical research · 2022Article
- A solid-supported membrane electrophysiology assay for efficient characterization of ion-coupled transport.The Journal of biological chemistry · 2021Article
- A GC-MS/Single-Cell Method to Evaluate Membrane Transporter Substrate Specificity and Signaling.Frontiers in molecular biosciences · 2021Article
- Solvent accessibility changes in a NaThe Journal of biological chemistry · 2020Article
- A kinetic mechanism for enhanced selectivity of membrane transport.PLoS computational biology · 2020Article
- EmrE reminds us to expect the unexpected in membrane transport.The Journal of general physiology · 2020Article
- Highly coupled transport can be achieved in free-exchange transport models.The Journal of general physiology · 2020Article
- Kinetic Models of Secondary Active Transporters.International journal of molecular sciences · 2019Article
- Article
- Models to determine the kinetic mechanisms of ion-coupled transporters.The Journal of general physiology · 2019Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The number of ions required to drive substrate transport through a secondary active transporter determines the protein's ability to create a substrate gradient, a feature essential to its physiological function, and places fundamental constraints on the transporter's mechanism. Stoichiometry is known for a wide array of mammalian transporters, but, due to a lack of readily available tools, not for most of the prokaryotic transporters for which high-resolution structures are available. Here, we describe a general method for using radiolabeled substrate flux assays to determine coupling stoichiometries of electrogenic secondary active transporters reconstituted in proteoliposomes by measuring transporter equilibrium potentials. We demonstrate the utility of this method by determining the coupling stoichiometry of VcINDY, a bacterial Na
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