Evidence map›Paper›PMID 28121156›Full record

ArticleJournal of proteome research2017

Assessment of Metabolic Changes in Mycobacterium smegmatis Wild-Type and alr Mutant Strains: Evidence of a New Pathway of d-Alanine Biosynthesis.

Darrell D Marshall, Steven Halouska, Denise K Zinniel, Robert J Fenton, Katie Kenealy, Harpreet K Chahal, Govardhan Rathnaiah, Raúl G Barletta, Robert Powers

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of proteome research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.9field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 12 citations in OpenAlex.

  1. Review
  2. International journal of molecular sciences · 2023
    Article
  3. Advances in Microbial NMR Metabolomics.Advances in experimental medicine and biology · 2023
    Article
  4. Article
  5. Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Darrell D MarshallDepartment of Chemistry, University of Nebraska-Lincoln , Lincoln, Nebraska 68588-0304, United States.
Steven HalouskaDepartment of Chemistry, University of Nebraska-Lincoln , Lincoln, Nebraska 68588-0304, United States.
Denise K ZinnielSchool of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln , Lincoln, Nebraska 68583-0905, United States.
Robert J FentonSchool of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln , Lincoln, Nebraska 68583-0905, United States.
Katie KenealySchool of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln , Lincoln, Nebraska 68583-0905, United States.
Harpreet K ChahalSchool of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln , Lincoln, Nebraska 68583-0905, United States.
Govardhan RathnaiahSchool of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln , Lincoln, Nebraska 68583-0905, United States.
Raúl G BarlettaSchool of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln , Lincoln, Nebraska 68583-0905, United States.
Robert PowersDepartment of Chemistry, University of Nebraska-Lincoln , Lincoln, Nebraska 68588-0304, United States.ORCID 0000-0001-9948-6837
University of Nebraska–Lincoln · US

Funding

THE REDOX-MEDIATED REGULATION OF IRON-RESPONSIVE GENE EXPRESSION IN LIVERP20RR017675 · NCRR · UNIVERSITY OF NEBRASKA LINCOLN · PI GRASS, GREGOR · 2002 to 2011
$20.4M
Redox Biology CenterP30GM103335 · NIGMS · UNIVERSITY OF NEBRASKA LINCOLN · PI BECKER, DONALD F · 2012 to 2016
$4.3M
EXTRAMURAL RESEARCH FACILITIES CONSTRUCTIONC06RR015468 · NCRR · UNIVERSITY OF NEBRASKA LINCOLN · PI PAUL, PREM S · 2000 to 2000
$2.0M
Citric acid cycle regulation of exopolysaccharide synthesis in staphylococciR01AI087668 · NIAID · UNIVERSITY OF NEBRASKA LINCOLN · PI SOMERVILLE, GREG ALAN · 2010 to 2013
$1.4M
Essentiality of Mycobacterium tuberculosis D-alanine racemaseR21AI087561 · NIAID · UNIVERSITY OF NEBRASKA LINCOLN · PI CHACON, OFELIA · 2010 to 2011
$393k
NCRR NIH HHS C06 RR015468NCRR NIH HHS P20 RR017675NIAID NIH HHS R01 AI087668NIAID NIH HHS R21 AI087561NIGMS NIH HHS P30 GM103335
6 · The paper itself

Abstract

In mycobacteria, d-alanine is an essential precursor for peptidoglycan biosynthesis. The only confirmed enzymatic pathway to form d-alanine is through the racemization of l-alanine by alanine racemase (Alr, EC 5.1.1.1). Nevertheless, the essentiality of Alr in Mycobacterium tuberculosis and Mycobacterium smegmatis for cell survivability in the absence of d-alanine has been a point of controversy with contradictory results reported in the literature. To address this issue, we examined the effects of alr inactivation on the cellular metabolism of M. smegmatis. The M. smegmatis alr insertion mutant TAM23 exhibited essentially identical growth to wild-type mc

Indexed as

Metabolic Networks and PathwaysAlanineAlanine RacemaseBacterial ProteinsMutationMycobacterium smegmatisPeptidoglycanTransaminasesAlanineAlanine RacemaseBacterial ProteinsPeptidoglycanTransaminasesalanine racemased-alanine biosynthesisMycobacterium smegmatisMycobacterium tuberculosisNMR metabolomics

Identifiers

PMID28121156
PMCPMC6599645
OpenAlexW2581610548

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.