Evidence map›Paper›PMID 28119952›Full record

ArticleData in brief2017

Data supporting the effects of lysozyme on mRNA and protein expression in a colonic epithelial scratch wound model.

Sarah K Abey, Yuana Yuana, Paule V Joseph, Natnael D Kenea, Nicolaas H Fourie, LeeAnne B Sherwin, Gregory E Gonye, Paul A Smyser, Erin S Stempinski, Christina M Boulineaux and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Data in brief, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 5 institutions in 2 countries.

Sarah K AbeyDigestive Disorders Unit, Division of Intramural Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA.
Yuana YuanaImage Sciences Institute, Division of Imaging, University Medical Centre Utrecht, Netherlands.
Paule V JosephDigestive Disorders Unit, Division of Intramural Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA.
Natnael D KeneaDigestive Disorders Unit, Division of Intramural Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA.
Nicolaas H FourieDigestive Disorders Unit, Division of Intramural Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA.
LeeAnne B SherwinDigestive Disorders Unit, Division of Intramural Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA.
Gregory E GonyeNanoString Technologies, Seattle, WA, USA.
Paul A SmyserThe Pennsylvania State University, College of Medicine, Hershey, PA, USA.
Erin S StempinskiElectron Microscopy Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA.
Christina M BoulineauxDigestive Disorders Unit, Division of Intramural Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA.
Kristen R WeaverDigestive Disorders Unit, Division of Intramural Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA.
Christopher K E BleckElectron Microscopy Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA.
Wendy A HendersonDigestive Disorders Unit, Division of Intramural Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD, USA.
National Institutes of Health · USNational Heart Lung and Blood Institute · USNanostring Technologies (United States) · USPennsylvania State University · USUniversity Medical Center Utrecht · NL

Funding

Electron Microscopy CoreZICHL005906 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI SYED, ZULFEQHAR · 2009 to 2025
$12.9M
NINR Intramural Research Training ProgramsZIENR000016 · NINR · NATIONAL INSTITUTE OF NURSING RESEARCH · PI TAMEZ, PAMELA · 2009 to 2019
$7.1M
Symptom Distress Mechanisms in Digestive DisordersZIANR000018 · NINR · NATIONAL INSTITUTE OF NURSING RESEARCH · PI HENDERSON, WENDY · 2009 to 2019
$5.2M
Sensory Science and Metabolism; Molecular and Neuronal MechanismsZIANR000035 · NINR · NATIONAL INSTITUTE OF NURSING RESEARCH · PI JOSEPH, PAULE · 2018 to 2020
$1.7M
Intramural NIH HHS ZIA NR000018
6 · The paper itself

Abstract

Colonic epithelial health is implicated in a host of gastrointestinal (GI) diseases and disorders. Lysozyme is suspected to play a role in the ability of the epithelium to recover from injury (Abey et al., in press; Gallo, 2012; Rubio, 2014) [1], [2], [3]. Disrupted repair mechanisms may lead to delayed or ineffective recovery and disruptions to epithelial biology resulting in GI symptoms and altered barrier function (Peterson and Artis, 2014) [4]. The effect of lysozyme on the transcriptomic and proteomic profile of healthy colonic epithelial cells was investigated. Epithelial cells in culture were scratch wounded and treated with lysozyme. mRNA and protein profiles were simultaneously quantified in the same sample using a digital counting technology. Gene and protein expressions altered by the presence or absence of lysozyme are described in this article. Extensive statistical and bioinformatic analysis, and interpretation of the results can be found in "Lysozyme association with circulating RNA, extracellular vesicles, and chronic stress" (Abey et al., in press) [1].

Identifiers

PMID28119952
PMCPMC5238358
OpenAlexW2563774654

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.