ArticleNucleic acids research2017
Nucleoid and cytoplasmic localization of small RNAs in Escherichia coli.
Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 19 citations in OpenAlex.
- From environmental signals to adaptive phenotypes: signal-responsive regulation and network logic of bacterial small RNAs.FEMS microbiology reviews · 2026Review
- A Safe and Versatile Minicell Platform Derived fromJournal of microbiology and biotechnology · 2025Article
- Manipulating subcellular protein localization to enhance target protein accumulation in minicells.Journal of biological engineering · 2025Article
- Bacterial nucleoid is a riddle wrapped in a mystery inside an enigma.Journal of bacteriology · 2024Review
- Analysis of Osmotic Tolerance, Physiological Characteristics, and Gene Expression ofACS omega · 2023Article
- Lipid-driven condensation and interfacial ordering of FUS.Science advances · 2022Article
- Three Microbial Musketeers of the Seas:International journal of molecular sciences · 2022Article
- Kinetic modeling reveals additional regulation at co-transcriptional level by post-transcriptional sRNA regulators.Cell reports · 2021Article
- Subcellular Architecture of themBio · 2021Article
- Adaptor protein RapZ activates endoribonuclease RNase E by protein-protein interaction to cleave a small regulatory RNA.RNA (New York, N.Y.) · 2020Article
- Coupled Transcription-Translation in Prokaryotes: An Old Couple With New Surprises.Frontiers in microbiology · 2020Review
- Unraveling the hidden universe of small proteins in bacterial genomes.Molecular systems biology · 2019Article
- EzColocalization: An ImageJ plugin for visualizing and measuring colocalization in cells and organisms.Scientific reports · 2018Article
- RNA Localization in Bacteria.Microbiology spectrum · 2018Review
- RNA search engines empower the bacterial intranet.Biochemical Society transactions · 2017Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial small RNAs (sRNAs) regulate protein production by binding to mRNAs and altering their translation and degradation. sRNAs are smaller than most mRNAs but larger than many proteins. Therefore it is uncertain whether sRNAs can enter the nucleoid to target nascent mRNAs. Here, we investigate the intracellular localization of sRNAs transcribed from plasmids in Escherichia coli using RNA fluorescent in-situ hybridization. We found that sRNAs (GlmZ, OxyS, RyhB and SgrS) have equal preference for the nucleoid and cytoplasm, and no preferential localization at the cell membrane. We show using the gfp mRNA (encoding green fluorescent protein) that non-sRNAs can be engineered to have different proportions of nucleoid and cytoplasmic localization by altering their length and/or translation. The same localization as sRNAs was achieved by decreasing gfp mRNA length and translation, which suggests that sRNAs and other RNAs may enter the densely packed DNA of the nucleoid if they are sufficiently small. We also found that the Hfq protein, which binds sRNAs, minimally affects sRNA localization. Important implications of our findings for engineering synthetic circuits are: (i) sRNAs can potentially bind nascent mRNAs in the nucleoid, and (ii) localization patterns and distribution volumes of sRNAs can differ from some larger RNAs.
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Registered trials
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