ArticleMetabolic engineering2017
Towards improved butanol production through targeted genetic modification of Clostridium pasteurianum.
Article in Metabolic engineering, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- A Consecutive Genome Engineering Method Reveals a New Phenotype and Regulation of Glucose and Glycerol Utilization inEngineering in life sciences · 2025Article
- The potential of native and engineered Clostridia for biomass biorefining.Frontiers in bioengineering and biotechnology · 2024Review
- A Review on Opportunities and Limitations of Membrane Bioreactor Configuration in Biofuel Production.Applied biochemistry and biotechnology · 2023Review
- Main genome characteristics of butanol-producing Clostridium sp. UCM В-7570 strain.Journal of applied genetics · 2023Article
- Single and multiplexed gene repression in solventogenicSynthetic and systems biotechnology · 2023Article
- Production of propionate using metabolically engineered strains of Clostridium saccharoperbutylacetonicum.Applied microbiology and biotechnology · 2022Article
- Production of butanol from lignocellulosic biomass: recent advances, challenges, and prospects.RSC advances · 2022Review
- Recent Advancements in the Technologies Detecting Food Spoiling Agents.Journal of functional biomaterials · 2021Review
- Renewable fatty acid ester production in Clostridium.Nature communications · 2021Article
- Article
- Sporulation in solventogenic and acetogenic clostridia.Applied microbiology and biotechnology · 2021Review
- Metabolomic and kinetic investigations on the electricity-aided production of butanol byEngineering in life sciences · 2021Article
- Development ofFrontiers in bioengineering and biotechnology · 2021Article
- Developing Clostridia as Cell Factories for Short- and Medium-Chain Ester Production.Frontiers in bioengineering and biotechnology · 2021Review
- Synthetic Biology Tools for Genome and Transcriptome Engineering of SolventogenicFrontiers in bioengineering and biotechnology · 2020Review
- Production of butanol from biomass: recent advances and future prospects.Environmental science and pollution research international · 2019Review
- Improved electrocompetence and metabolic engineering ofEngineering in life sciences · 2019Article
- Role of the global regulator Rex in control of NADMolecular microbiology · 2019Article
- Recent Developments of the Synthetic Biology Toolkit forFrontiers in microbiology · 2018Review
- Improving gene transfer in Clostridium pasteurianum through the isolation of rare hypertransformable variants.Anaerobe · 2017Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Declining fossil fuel reserves, coupled with environmental concerns over their continued extraction and exploitation have led to strenuous efforts to identify renewable routes to energy and fuels. One attractive option is to convert glycerol, a by-product of the biodiesel industry, into n-butanol, an industrially important chemical and potential liquid transportation fuel, using Clostridium pasteurianum. Under certain growth conditions this Clostridium species has been shown to predominantly produce n-butanol, together with ethanol and 1,3-propanediol, when grown on glycerol. Further increases in the yields of n-butanol produced by C. pasteurianum could be accomplished through rational metabolic engineering of the strain. Accordingly, in the current report we have developed and exemplified a robust tool kit for the metabolic engineering of C. pasteurianum and used the system to make the first reported in-frame deletion mutants of pivotal genes involved in solvent production, namely hydA (hydrogenase), rex (Redox response regulator) and dhaBCE (glycerol dehydratase). We were, for the first time in C. pasteurianum, able to eliminate 1,3-propanediol synthesis and demonstrate its production was essential for growth on glycerol as a carbon source. Inactivation of both rex and hydA resulted in increased n-butanol titres, representing the first steps towards improving the utilisation of C. pasteurianum as a chassis for the industrial production of this important chemical.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.