ArticlePloS one2017
FTIR Spectroscopy for Evaluation and Monitoring of Lipid Extraction Efficiency for Oleaginous Fungi.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
58 citing papers in PubMed.
- Next-generation diabetes diagnostics: spectrochemical staging of red blood cells using vibrational spectroscopy.Journal of advanced research · 2026Article
- Exosome in Hashimoto's thyroiditis: from pathogenesis to diagnostic and therapeutic potential.Molecular biology reports · 2026Review
- Vibrational spectroscopy methodology for profiling of mixed carbon metabolism in carotenogenic and oleaginous Mucor circinelloides.Microbial cell factories · 2026Article
- Exploring allomelanin: A comparative analysis via natural product extraction and synthesis.Science advances · 2026Article
- Investigations of Diclofenac Sorption on Intact and ModifiedInternational journal of molecular sciences · 2026Article
- Application of vibrational spectroscopies as process analytical techniques for monitoring fermentation and the conversion of lignocellulosic biomass by oleaginous filamentous fungi.Microbial cell factories · 2025Article
- Circular valorization of Argemone ochroleuca seed meal: biomass fractionation, bioethanol production, and hydrothermal carbonization.Bioresources and bioprocessing · 2025Article
- Characterization and statistical lipid optimization of an oleaginous soil fungus, Gongronella butleri HMA-10: a promising novel source for sustainable lipid production.International microbiology : the official journal of the Spanish Society for Microbiology · 2025Article
- Calcium starvation leads to strain-specific gene regulation of lipid and carotenoid production in Mucor circinelloides.G3 (Bethesda, Md.) · 2025Article
- Diversity of culturable fungi in Antarctic lakes and their potential for producing compounds of biotechnological interest.Extremophiles : life under extreme conditions · 2025Article
- Article
- Insight into the Phase Inversion of Myristic Acid In Situ Gels for Drug Delivery Applications.ACS omega · 2025Article
- Biological, Biochemical and Elemental Traits ofAnimals : an open access journal from MDPI · 2025Article
- Lipid Production in Cultivable Filamentous Fungi Isolated from Antarctic Soils: A Comprehensive Study.Microorganisms · 2025Article
- Engineering pH and Temperature-Triggered Drug Release with Metal-Organic Frameworks and Fatty Acids.Molecules (Basel, Switzerland) · 2024Article
- Biovalorization of whey waste as economic nutriment for mycogenic production of single cell oils with promising antibiofilm and anticancer potentiality.Journal of biological engineering · 2024Article
- The Relationship Between the Growth ofReports of biochemistry & molecular biology · 2024Article
- Revisiting the activity of two poly(vinyl chloride)- and polyethylene-degrading enzymes.Nature communications · 2024Article
- Multifaceted Impact of Lipid Extraction on the Characteristics of Polymer-Based Sewage Sludge towards Sustainable Sludge Management.Polymers · 2024Article
- Explorative characterization and taxonomy-aligned comparison of alterations in lipids and other biomolecules in Antarctic bacteria grown at different temperatures.Environmental microbiology reports · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To assess whether Fourier Transform Infrared (FTIR) spectroscopy could be used to evaluate and monitor lipid extraction processes, the extraction methods of Folch, Bligh and Lewis were used. Biomass of the oleaginous fungi Mucor circinelloides and Mortierella alpina were employed as lipid-rich material for the lipid extraction. The presence of lipids was determined by recording infrared spectra of all components in the lipid extraction procedure, such as the biomass before and after extraction, the water and extract phases. Infrared spectra revealed the incomplete extraction after all three extraction methods applied to M.circinelloides and it was shown that mechanical disruption using bead beating and HCl treatment were necessary to complete the extraction in this species. FTIR spectroscopy was used to identify components, such as polyphosphates, that may have negatively affected the extraction process and resulted in differences in extraction efficiency between M.circinelloides and M.alpina. Residual lipids could not be detected in the infrared spectra of M.alpina biomass after extraction using the Folch and Lewis methods, indicating their complete lipid extraction in this species. Bligh extraction underestimated the fatty acid content of both M.circinelloides and M.alpina biomass and an increase in the initial solvent-to-sample ratio (from 3:1 to 20:1) was needed to achieve complete extraction and a lipid-free IR spectrum. In accordance with previous studies, the gravimetric lipid yield was shown to overestimate the potential of the SCO producers and FAME quantification in GC-FID was found to be the best-suited method for lipid quantification. We conclude that FTIR spectroscopy can serve as a tool for evaluating the lipid extraction efficiency, in addition to identifying components that may affect lipid extraction processes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.