Evidence map›Paper›PMID 28118377›Full record

ArticlePloS one2017

γH2Ax Expression as a Potential Biomarker Differentiating between Low and High Grade Cervical Squamous Intraepithelial Lesions (SIL) and High Risk HPV Related SIL.

Konstantinos Leventakos, Sotirios Tsiodras, Theodore Kelesidis, Maria Kefala, Christine Kottaridi, Aris Spathis, Alina-Roxani Gouloumi, Abraham Pouliakis, Asimakis Pappas, Vasileios Sioulas and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Expression of HPV-induced DNA Damage Repair Factors Correlates With CIN Progression.International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 1 institution in 1 country.

Konstantinos Leventakos2nd Department of Pathology, University Hospital "Attikon", School of Medicine, National and Kapodistrian University of Athens, Athens, Greece.
Sotirios Tsiodras4th Department of Internal Medicine, University Hospital "Attikon", School of Medicine, National and Kapodistrian University of Athens, Athens, Greece.ORCID http://orcid.org/0000-0002-0463-4321
Theodore Kelesidis4th Department of Internal Medicine, University Hospital "Attikon", School of Medicine, National and Kapodistrian University of Athens, Athens, Greece.
Maria Kefala2nd Department of Pathology, University Hospital "Attikon", School of Medicine, National and Kapodistrian University of Athens, Athens, Greece.
Christine KottaridiDepartment of Cytopathology, University Hospital "Attikon", School of Medicine, National and Kapodistrian University of Athens, Athens, Greece.
Aris SpathisDepartment of Cytopathology, University Hospital "Attikon", School of Medicine, National and Kapodistrian University of Athens, Athens, Greece.
Alina-Roxani Gouloumi2nd Department of Pathology, University Hospital "Attikon", School of Medicine, National and Kapodistrian University of Athens, Athens, Greece.
Abraham PouliakisDepartment of Cytopathology, University Hospital "Attikon", School of Medicine, National and Kapodistrian University of Athens, Athens, Greece.
Asimakis Pappas3rd Department of Obstetrics and Gynecology, University Hospital "Attikon", School of Medicine, National and Kapodistrian University of Athens, Athens, Greece.
Vasileios Sioulas3rd Department of Obstetrics and Gynecology, University Hospital "Attikon", School of Medicine, National and Kapodistrian University of Athens, Athens, Greece.
Charalambos Chrelias3rd Department of Obstetrics and Gynecology, University Hospital "Attikon", School of Medicine, National and Kapodistrian University of Athens, Athens, Greece.
Petros KarakitsosDepartment of Cytopathology, University Hospital "Attikon", School of Medicine, National and Kapodistrian University of Athens, Athens, Greece.
Ioannis Panayiotides2nd Department of Pathology, University Hospital "Attikon", School of Medicine, National and Kapodistrian University of Athens, Athens, Greece.
National and Kapodistrian University of Athens · GR

Funding

UCLA Clinical and Translational Science InstituteUL1TR000124 · NCATS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI DUBINETT, STEVEN M. · 2012 to 2015
$57.0M
Oxidized HDL in the intersection of HIV, immune activation and atherosclerosisK08AI108272 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI KELESIDIS, THEODOROS · 2013 to 2017
$915k
NCATS NIH HHS UL1 TR000124NIAID NIH HHS K08 AI108272
6 · The paper itself

Abstract

backgroundγH2AX is a protein biomarker for double-stranded DNA breakage; its expression was studied in cervical squamous intraepithelial lesions and carcinomas.

methodsImmunostaining for phospho-γH2AX was performed in sections from histologically confirmed cervical SIL and carcinomas, as well as from normal cervices used as controls. In total, 275 cases were included in the study: 112 low grade SIL (LGSIL), 99 high grade SIL (HGSIL), 24 squamous cell carcinoma (SCC), 12 adenocarcinoma and 28 cervical specimens with no essential lesions. Correlation of histological grading, high risk vs. low risk HPV virus presence, activated vs. non-activated status (by high risk HPV mRNA expression) and γH2AX expression in both basal and surface segments of the squamous epithelium was performed.

resultsGradual increase of both basal and surface γH2AX expression was noted up from normal cervices to LGSIL harboring a low risk HPV type, to LGSIL harboring a high risk virus at a non-activated state (p<0.05). Thereafter, both basal and surface γH2AX expression dropped in LGSIL harboring a high risk virus at an activated state and in HGSIL.

conclusionsγH2AX could serve as a potential biomarker discriminating between LGSIL and HGSIL, as well as between LGSIL harboring high risk HPV at an activated state.

Indexed as

AdenocarcinomaAdolescentAdultAgedAged, 80 and overBiomarkers, TumorCarcinoma, Squamous CellCervix UteriDiagnosis, DifferentialEpithelial CellsFemaleHistonesHuman Papillomavirus DNA TestsHumansMiddle AgedNeoplasm GradingBiomarkers, TumorH2AX protein, humanHistonesNeoplasm Proteins

Identifiers

PMID28118377
PMCPMC5261776
OpenAlexW2582615201

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.