Evidence map›Paper›PMID 28117948›Full record

ArticleInternational forum of allergy & rhinology2017

Bactericidal antibiotics promote oxidative damage and programmed cell death in sinonasal epithelial cells.

Michael A Kohanski, Anuj Tharakan, Nyall R London, Andrew P Lane, Murugappan Ramanathan

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International forum of allergy & rhinology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.8field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 15 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Article
  5. Article
  6. Article
  7. Antibiotics Affect ROS Production and Fibroblast Migration in anFrontiers in cellular and infection microbiology · 2020
    Article
  8. Impact of antibiotics on smell dysfunction.World journal of otorhinolaryngology - head and neck surgery · 2018
    Article
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Michael A KohanskiDepartment of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, MD.
Anuj TharakanDepartment of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, MD.
Nyall R LondonDepartment of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, MD.
Andrew P LaneDepartment of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, MD.
Murugappan RamanathanDepartment of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, MD.
Johns Hopkins Medicine · USJohns Hopkins University · US

Funding

Immune Responses of the Epithelium in Chronic Rhinosinusitis with PolypsR01AI072502 · NIAID · JOHNS HOPKINS UNIVERSITY · PI LANE, ANDREW P · 2007 to 2016
$3.9M
Role of the Indoor Environment on Oxidative Stress Related Sinonasal InflammationK23ES020859 · NIEHS · JOHNS HOPKINS UNIVERSITY · PI RAMANATHAN, MURUGAPPAN · 2011 to 2015
$1.2M
NIAID NIH HHS R01 AI072502NIEHS NIH HHS K23 ES020859NIEHS NIH HHS L30 ES022003
6 · The paper itself

Abstract

backgroundAntibiotics are widely and heavily used in the treatment of chronic sinusitis. Bactericidal antibiotics can stimulate reactive oxygen species (ROS) formation, a proinflammatory response, and cell death in cultured human sinonasal epithelial cells (SNECs). Sulforaphane (SFN) is a potent stimulator of the antioxidant nuclear factor erythroid 2-related factor 2 (Nrf-2) system and a suppressor of inflammation. In this study we utilized SFN to further explore the relationship between levofloxacin treatment, ROS formation, and the cell death response.

methodsSNECs were collected from patients during endoscopic sinus surgery and grown in culture at the air-liquid interface. Differentiated SNECs were stimulated with levofloxacin with or without SFN pretreatment. ROS were quantified. Apoptosis markers of caspase-3 activity and DNA fragmentation were quantified.

resultsCultured SNECs treated with levofloxacin resulted in a significant increase in activity of the proapoptotic caspase-3 protease (5.9-fold, p = 0.01). The increase in activity was suppressed by pretreatment with SFN (1.9-fold). ROS levels increased with levofloxacin treatment (range, 1.2-fold to 1.8-fold), but were not significantly suppressed by pretreatment with SFN (range, 1.0-fold to 1.3-fold).

conclusionIn this study, we demonstrate that treatment of cultured SNECs with levofloxacin leads to an increase in caspase-3 activity. SFN pretreatment suppresses the increased apoptotic response possibly through its antioxidant stimulating properties. Our results suggest that levofloxacin treatment stimulates a potent proapoptotic possibly through an ROS-dependent mechanism. Future studies will explore if this antibiotic-induced response is harmful to recovery of function in those with sinusitis.

Indexed as

Anti-Bacterial AgentsAntioxidantsApoptosisCaspase 3Cells, CulturedDNA DamageEpithelial CellsEthmoid SinusHumansIsothiocyanatesLevofloxacinOxidative StressReactive Oxygen SpeciesSulfoxidesAnti-Bacterial AgentsAntioxidantsCaspase 3IsothiocyanatesLevofloxacinReactive Oxygen SpeciessulforaphaneSulfoxidesantibioticsapoptosisinnate immunityrhinosinusitisROSsulforaphane

Identifiers

PMID28117948
PMCPMC5386800
OpenAlexW2581250649

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.