ArticleJournal of visualized experiments : JoVE2017
Tunable Hydrogels from Pulmonary Extracellular Matrix for 3D Cell Culture.
Article in Journal of visualized experiments : JoVE, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 33 citations in OpenAlex.
- Decellularized murine lung and brain provide matrix material for tissue culture studies.MethodsX · 2026Article
- Tailored Levofloxacin Incorporated Extracellular Matrix Nanoparticles for Pulmonary Infections.International journal of molecular sciences · 2025Article
- Tissue-Informed Biomaterial Innovations Advance Pulmonary Regenerative Engineering.ACS macro letters · 2025Review
- Binary fabrication of decellularized lung extracellular matrix hybridgels for in vitro chronic obstructive pulmonary disease modeling.Acta biomaterialia · 2024Article
- Cryosectioning of Hydrogels as a Reliable Approach to Increase Yield and Further Tune Mechanical Properties.Gels (Basel, Switzerland) · 2023Article
- Human alveolar hydrogels promote morphological and transcriptional differentiation in iPSC-derived alveolar type 2 epithelial cells.Scientific reports · 2023Article
- Regional and disease specific human lung extracellular matrix composition.Biomaterials · 2023Article
- Engineering Dynamic 3D Models of Lung.Advances in experimental medicine and biology · 2023Article
- Using extracellular matrix derived from sugen-chronic hypoxia lung tissue to study pulmonary arterial hypertension.Frontiers in pharmacology · 2023Article
- Current possibilities and future opportunities provided by three-dimensional lung ECM-derived hydrogels.Frontiers in pharmacology · 2023Article
- Ordered micropattern arrays fabricated by lung-derived dECM hydrogels for chemotherapeutic drug screening.Materials today. Bio · 2022Article
- Multifunctional and Self-Healable Intelligent Hydrogels for Cancer Drug Delivery and Promoting Tissue Regeneration In Vivo.Polymers · 2021Review
- Bioprintable Lung Extracellular Matrix Hydrogel Scaffolds for 3D Culture of Mesenchymal Stromal Cells.Polymers · 2021Article
- A 3D Bioprinted Material That Recapitulates the Perivascular Bone Marrow Structure for Sustained Hematopoietic and Cancer Models.Polymers · 2021Article
- A Two-Step Bioreactor for Decellularized Lung Epithelialization.Cells, tissues, organs · 2021Article
- A Natural Xenogeneic Endometrial Extracellular Matrix Hydrogel Toward Improving Current HumanFrontiers in bioengineering and biotechnology · 2021Article
- Porcine Lung-Derived Extracellular Matrix Hydrogel Properties Are Dependent on Pepsin Digestion Time.Tissue engineering. Part C, Methods · 2020Article
- Laminin-driven Epac/Rap1 regulation of epithelial barriers on decellularized matrix.Acta biomaterialia · 2019Article
- Studying Normal Tissue Radiation Effects using Extracellular Matrix Hydrogels.Journal of visualized experiments : JoVE · 2019Article
- Extracellular matrix hydrogel therapies: In vivo applications and development.Acta biomaterialia · 2018Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Here we present a method for establishing multiple component cell culture hydrogels for in vitro lung cell culture. Beginning with healthy en bloc lung tissue from porcine, rat, or mouse, the tissue is perfused and submerged in subsequent chemical detergents to remove the cellular debris. Histological comparison of the tissue before and after processing confirms removal of over 95% of double stranded DNA and alpha galactosidase staining suggests the majority of cellular debris is removed. After decellularization, the tissue is lyophilized and then cryomilled into a powder. The matrix powder is digested for 48 hr in an acidic pepsin digestion solution and then neutralized to form the pregel solution. Gelation of the pregel solution can be induced by incubation at 37 °C and can be used immediately following neutralization or stored at 4 °C for up to two weeks. Coatings can be formed using the pregel solution on a non-treated plate for cell attachment. Cells can be suspended in the pregel prior to self-assembly to achieve a 3D culture, plated on the surface of a formed gel from which the cells can migrate through the scaffold, or plated on the coatings. Alterations to the strategy presented can impact gelation temperature, strength, or protein fragment sizes. Beyond hydrogel formation, the hydrogel stiffness may be increased using genipin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.