ArticleScientific reports2017
Selection of reference genes is critical for miRNA expression analysis in human cardiac tissue. A focus on atrial fibrillation.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers, 2 of them syntheses that pooled it.
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Who cites it
55 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- microRNA signatures associated with fetal growth restriction: a systematic review.European journal of clinical nutrition · 2022Pooled it
- MicroRNAs in Atrial Fibrillation: from Expression Signatures to Functional Implications.Cardiovascular drugs and therapy · 2017Pooled it
- Identification of stable miRNA references for qRT-PCR normalization in ovine fasciolosis.BMC genomics · 2026Article
- Expression levels of miR-132, miR-134, and miR-195 in treatment-naïve children and adolescents with obsessive-compulsive disorder: a case-control study.BMC psychiatry · 2026Article
- MicroRNA expression profile associated with coronary heart disease in patients with resistant arterial hypertension.Frontiers in molecular biosciences · 2026Article
- Application of microRNA In Situ Hybridization on Long-term Stored Human Formalin-fixed Paraffin-embedded Brain Samples from Psychiatric Patients.Molecular neurobiology · 2025Article
- Exploring the Role of microRNAs as Blood Biomarkers in Alzheimer's Disease and Frontotemporal Dementia.International journal of molecular sciences · 2025Review
- Influence of Reference Gene Selection on miRNA Quantification by RT-qPCR in Human Placental Samples.British journal of biomedical science · 2025Article
- NinoaPathogens (Basel, Switzerland) · 2024Article
- MicroRNAs, endometrial receptivity and molecular pathways.Reproductive biology and endocrinology : RB&E · 2024Review
- Benchmarking miRNA reference genes in B-cell precursor acute lymphoblastic leukemia.Scientific reports · 2024Article
- Identification of the optimal reference genes for atrial fibrillation model established by iPSC-derived atrial myocytes.BMC genomics · 2024Article
- Extracellular vesicles from human cardiac stromal cells up-regulate cardiomyocyte protective responses to hypoxia.Stem cell research & therapy · 2024Article
- Absolute Quantification of Selected microRNAs Expression in Endometrial Cancer by Digital PCR.International journal of molecular sciences · 2024Article
- Review
- Dysregulation of miR-122, miR-574 and miR-375 in Egyptian patients with breast cancer.PloS one · 2024Article
- MicroRNAs in atrial fibrillation target genes in structural remodelling.Cell and tissue research · 2023Article
- MiR-423-5p as Optimal Endogenous Control for Quantification of Circulating MicroRNAs in Patients With CKD.Kidney international reports · 2023Article
- miRNAs signature as potential biomarkers for cervical precancerous lesions in human papillomavirus positive women.Scientific reports · 2023Article
- Screening of reference genes for microRNA analysis in the study of solider caste differentiation of Formosan subterranean termite Coptotermes formosanus Shiraki.Scientific reports · 2023Article
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8 authors.
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No grant is acknowledged in the PubMed record.
Abstract
MicroRNAs (miRNAs) are emerging as key regulators of complex biological processes in several cardiovascular diseases, including atrial fibrillation (AF). Reverse transcription-quantitative polymerase chain reaction is a powerful technique to quantitatively assess miRNA expression profile, but reliable results depend on proper data normalization by suitable reference genes. Despite the increasing number of studies assessing miRNAs in cardiac disease, no consensus on the best reference genes has been reached. This work aims to assess reference genes stability in human cardiac tissue with a focus on AF investigation. We evaluated the stability of five reference genes (U6, SNORD48, SNORD44, miR-16, and 5S) in atrial tissue samples from eighteen cardiac-surgery patients in sinus rhythm and AF. Stability was quantified by combining BestKeeper, delta-C
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