Anomalous Separation of Small Y-Chromosomal DNA Fragments on Microchip Electrophoresis.

Mohammad Jabasini, Ashraf Ewis, Youichi Sato, Yutaka Nakahori, Yoshinobu Baba

Abstract read

In one paragraph

Article in Scientia pharmaceutica, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Mohammad JabasiniDepartment of Pharmaceutical Information Science, Institute of Health Biosciences, The University of Tokushima, 1-78-1 Sho-machi, 770-8505 Tokushima, Japan. jabasini1@gmail.com.

Ashraf EwisDepartment of Human Genetics Public Health, School of Medicine, The University of Tokushima, 3-18-15 Kuramoto-cho, 770-8503 Tokushima, Japan. ashraf_ewis@yahoo.com.

Youichi SatoDepartment of Pharmaceutical Information Science, Institute of Health Biosciences, The University of Tokushima, 1-78-1 Sho-machi, 770-8505 Tokushima, Japan. sato@ph.tokushima-u.ac.jp.

Yutaka NakahoriDepartment of Human Genetics Public Health, School of Medicine, The University of Tokushima, 3-18-15 Kuramoto-cho, 770-8503 Tokushima, Japan. nakahori@basic.med.tokushima-u.ac.jp.

Yoshinobu BabaDepartment of Applied Chemistry, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, 464-8603 Nagoya, Japan. babaymtt@apchem.nagoya-u.ac.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We investigated an anomalous DNA separation where two DNA fragments from the human Y-chromosome sY638 (64 bp) and sY592 (65 bp), with only one base pair difference, were separated. This result is abnormal since in a previous study, we found that 5 bp was the minimum difference between two DNA fragments that the microchip electrophoresis system can separate. The formation of a mini-loop in the structure of the DNA fragment of sY638 (64 bp) was strongly expected to be the reason. To investigate this, we synthesized three modified DNA fragments for sY638 (64 bp), and the modifications were in two expected locations for possible mini-loop formation. Later, the separation between sY592 (65 bp) and the three modified fragments of sY638 (64 bp) was not possible. Thus, we conclude that the formation of a mini-loop in the structure of the DNA is the reason behind this anomalous separation.

Indexed as

anomalous separationDNAmicrochip electrophoresis

Identifiers

PMID28117316

DOI10.3390/scipharm84030507

PMCPMC5064241

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.