ArticleJournal of pharmaceutical health care and sciences2017
Comparison of 16S ribosomal RNA gene sequence analysis and conventional culture in the environmental survey of a hospital.
Article in Journal of pharmaceutical health care and sciences, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- The Potential of Bioinformatics in Revolutionizing Disease Diagnosis in Sub-Saharan Africa: A Narrative Review.Health science reports · 2026Article
- Microbial species in deep tissue lesions of Hurley stage III hidradenitis suppurativa: a retrospective observational study.BMC microbiology · 2026Observational
- Pumping and hygiene practices are associated with bacterial load and microbial composition in human milk expressed at home.Journal of translational medicine · 2025Article
- Microbial Community Characterization and Molecular Resistance Monitoring in Geriatric Intensive Care Units in China Using mNGS.Infection and drug resistance · 2023Article
- Global airborne bacterial community-interactions with Earth's microbiomes and anthropogenic activities.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- The Effect of Radiation and Chemoradiation Therapy on the Head and Neck Mucosal Microbiome: A Review.Frontiers in oncology · 2021Review
- Isolation of Gram-Positive, Antibiotic-Resistant Bacteria from Tactile Mobile Phones in a Northwestern Mexican City.Journal of community health · 2020Article
- Systematic processing of ribosomal RNA gene amplicon sequencing data.GigaScience · 2019Article
- Article
- 16SPIP: a comprehensive analysis pipeline for rapid pathogen detection in clinical samples based on 16S metagenomic sequencing.BMC bioinformatics · 2017Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNosocomial infection is one of the most common complications within health care facilities. Certain studies have reported outbreaks resulting from contaminated hospital environments. Although the identification of bacteria in the environment can readily be achieved using culturing methods, these methods detect live bacteria. Sequencing of the 16S ribosomal RNA (16S rRNA) gene is recognized to be effective for bacterial identification. In this study, we surveyed wards where drug-resistant bacteria had been isolated and compared conventional culture methods with 16S rRNA gene sequencing methods.
methodsSamples were collected using sterile swabs from two wards (northern and southern) at Gunma University Hospital contaminated by
resultsIn the northern ward,
conclusionsMolecular biological analysis showed a higher sensitivity to detect specific bacteria and detected a greater number of species than the culture method. Our results suggest that 16S rRNA gene sequencing analysis is useful to identify range of contamination which were not found in conventional culture method. When a nosocomial outbreak cannot be adequately controlled, molecular biological analysis may serve as a useful tool for environmental surveys in hospitals.
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