ArticleProceedings of the National Academy of Sciences of the United States of America2017
Heat-induced masculinization in domesticated zebrafish is family-specific and yields a set of different gonadal transcriptomes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 137 citations in OpenAlex.
- Novel Method for Preparing Pseudo-Male Common Carp by Inducing Secondary Female-to-Male Sex Reversal Under High Temperature Conditions.Marine biotechnology (New York, N.Y.) · 2026Article
- Multigenerational temperature effects on paternal epigenetic inheritance in European sea bass.Epigenetics & chromatin · 2025Article
- Article
- Identification of sex-biased MiRNA markers informative of heat-past events.BMC genomics · 2025Article
- Zglp-1 is a novel essential transcriptional regulator for sex reversal in zebrafish.Marine life science & technology · 2025Article
- Sexual dimorphism in the DNA methylation pattern after immune stimulation in the zebrafish (Frontiers in cell and developmental biology · 2025Article
- Adamts9 is required for the development of primary ovarian follicles and maintenance of female sex in zebrafish†.Biology of reproduction · 2024Article
- Extraordinary variability in gene activation and repression programs during gonadal sex differentiation across vertebrates.Frontiers in cell and developmental biology · 2024Article
- Article
- Exploring the Effects of Rearing Densities on Epigenetic Modifications in the Zebrafish Gonads.International journal of molecular sciences · 2023Article
- The use ofSystems biology in reproductive medicine · 2023Article
- Development of epigenetic biomarkers for the identification of sex and thermal stress in fish using DNA methylation analysis and machine learning procedures.Molecular ecology resources · 2023Article
- Effect of high temperatures on sex ratio and differential expression analysis (RNA-seq) of sex-determining genes inEnvironmental epigenetics · 2023Article
- A Simple Method for Inducing Masculinization of Zebrafish Stocks Using 17α-Methyltestosterone.Zebrafish · 2022Review
- Deciphering sex-specific miRNAs as heat-recorders in zebrafish.Scientific reports · 2022Article
- Environmentally-induced sex reversal in fish with chromosomal vs. polygenic sex determination.Environmental research · 2022Article
- Improved biomarker discovery through a plot twist in transcriptomic data analysis.BMC biology · 2022Article
- Comparative transcriptome analysis of heat-induced domesticated zebrafish during gonadal differentiation.BMC genomic data · 2022Article
- Gonadal bacterial community composition is associated with sex-specific differences in swamp eelsFrontiers in immunology · 2022Article
- Comparative Transcriptome Analyses Characterize Expression Signatures Among Males, Females, Neo-Males, and Gynogenetic Females in the Yellow Drum (Frontiers in genetics · 2022Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Understanding environmental influences on sex ratios is important for the study of the evolution of sex-determining mechanisms and for evaluating the effects of global warming and chemical pollution. Fishes exhibit sexual plasticity, but the underlying mechanisms of environmental effects on their reproduction are unclear even in the well-established teleost research model, the zebrafish. Here we established the conditions to study the effects of elevated temperature on zebrafish sex. We showed that sex ratio response to elevated temperature is family-specific and typically leads to masculinization (female-to-male sex reversal), resulting in neomales. These results uncovered genotype-by-environment interactions that support a polygenic sex determination system in domesticated (laboratory) zebrafish. We found that some heat-treated fish had gene expression profiles similar to untreated controls of the same sex, indicating that they were resistant to thermal effects. Further, most neomales had gonadal transcriptomes similar to that of regular males. Strikingly, we discovered heat-treated females that displayed a normal ovarian phenotype but with a "male-like" gonadal transcriptome. Such major transcriptomic reprogramming with preserved organ structure has never been reported. Juveniles were also found to have a male-like transcriptome shortly after exposure to heat. These findings were validated by analyzing the expression of genes and signaling pathways associated with sex differentiation. Our results revealed a lasting thermal effect on zebrafish gonads, suggesting new avenues for detection of functional consequences of elevated temperature in natural fish populations in a global warming scenario.
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