ArticleVirology journal2017
Host Cell Copper Transporters CTR1 and ATP7A are important for Influenza A virus replication.
Article in Virology journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 51 citations in OpenAlex.
- Enhancing immunity in viral infections, with special emphasis on COVID-19: A review.Diabetes & metabolic syndromePooled it
- Copper-mediated MAM regulation of the NF-κB signalling pathway enhances Seneca Valley virus replication in PK-15 cells.Veterinary research · 2025Article
- Integrating cuproptosis and immunosenescence: A novel therapeutic strategy in cancer treatment.Biochemistry and biophysics reports · 2025Review
- Leishmania major-induced alteration of host cellular and systemic copper homeostasis drives the fate of infection.Communications biology · 2024Article
- The Molecular Mechanisms of Cuproptosis and Small-Molecule Drug Design in Diabetes Mellitus.Molecules (Basel, Switzerland) · 2024Review
- Non-Negligible Role of Trace Elements in Influenza Virus Infection.Metabolites · 2023Review
- The interplay between copper metabolism and microbes: in perspective of host copper-dependent ATPases ATP7A/B.Frontiers in cellular and infection microbiology · 2023Review
- Article
- Alternative splicing liberates a cryptic cytoplasmic isoform of mitochondrial MECR that antagonizes influenza virus.PLoS biology · 2022Article
- Testing of a mobile heating facility to sanitize N-95 respirators against an enveloped respiratory virus.International journal of circumpolar health · 2022Article
- Exploring the Role of Heavy Metals and Their Derivatives on the Pathophysiology of COVID-19.Biological trace element research · 2022Review
- The Crossroads between Host Copper Metabolism and Influenza Infection.International journal of molecular sciences · 2021Review
- Herbal immune-boosters: Substantial warriors of pandemic Covid-19 battle.Phytomedicine : international journal of phytotherapy and phytopharmacology · 2021Review
- Nutritional Impact and Its Potential Consequences on COVID-19 Severity.Frontiers in nutrition · 2021Review
- Kidney diseases and COVID-19 infection: causes and effect, supportive therapeutics and nutritional perspectives.Heliyon · 2021Review
- Obesity, malnutrition, and trace element deficiency in the coronavirus disease (COVID-19) pandemic: An overview.Nutrition (Burbank, Los Angeles County, Calif.) · 2021Review
- Anti-Influenza Effect of Nanosilver in a Mouse Model.Vaccines · 2020Article
- Host - virus - drug interactions as determinants of COVID-19's phenotypes: A data-driven hypothesis.Medical hypotheses · 2020Article
- COVID-19: The Potential Role of Copper and N-acetylcysteine (NAC) in a Combination of Candidate Antiviral Treatments Against SARS-CoV-2.In vivo (Athens, Greece) · 2020Review
- nCOVID-19 Pandemic: From Molecular Pathogenesis to Potential Investigational Therapeutics.Frontiers in cell and developmental biology · 2020Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundThe essential role of copper in eukaryotic cellular physiology is known, but has not been recognized as important in the context of influenza A virus infection. In this study, we investigated the effect of cellular copper on influenza A virus replication.
methodsInfluenza A/WSN/33 (H1N1) virus growth and macromolecule syntheses were assessed in cultured human lung cells (A549) where the copper concentration of the growth medium was modified, or expression of host genes involved in copper homeostasis was targeted by RNA interference.
resultsExogenously increasing copper concentration, or chelating copper, resulted in moderate defects in viral growth. Nucleoprotein (NP) localization, neuraminidase activity assays and transmission electron microscopy did not reveal significant defects in virion assembly, morphology or release under these conditions. However, RNAi knockdown of the high-affinity copper importer CTR1 resulted in significant viral growth defects (7.3-fold reduced titer at 24 hours post-infection, p = 0.04). Knockdown of CTR1 or the trans-Golgi copper transporter ATP7A significantly reduced polymerase activity in a minigenome assay. Both copper transporters were required for authentic viral RNA synthesis and NP and matrix (M1) protein accumulation in the infected cell.
conclusionsThese results demonstrate that intracellular copper regulates the influenza virus life cycle, with potentially distinct mechanisms in specific cellular compartments. These observations provide a new avenue for drug development and studies of influenza virus pathogenesis.
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