ArticlePloS one2017
Contribution of the Pseudomonas fluorescens MFE01 Type VI Secretion System to Biofilm Formation.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 74 citations in OpenAlex.
- Functional diversity of accessory factors of the type VI secretion system.Journal of bacteriology · 2026Review
- From past to present: the crosstalk between type VI secretion system of Salmonella and host.Archives of microbiology · 2025Review
- ARTP mutagenesis for genome-wide identification of genes important for biofilm regulation in spoilage bacteriumApplied and environmental microbiology · 2025Article
- The GacS/GacA two-component system strongly regulates antimicrobial competition mechanisms ofJournal of bacteriology · 2025Article
- Identification of a TonB-Dependent Siderophore Receptor as a Novel Anti-Biofilm Target and Virtual Screening for Its Inhibitor inFoods (Basel, Switzerland) · 2025Article
- AcsS Negatively Regulates the Transcription of type VI Secretion System 2 Genes in Vibrio parahaemolyticus.Current microbiology · 2024Article
- Molecular characterization and in-depth genomic analysis to unravel the pathogenic features of an environmental isolate Enterobacter sp. S-33.International microbiology : the official journal of the Spanish Society for Microbiology · 2024Article
- IcmF2 of the type VI secretion system 2 plays a role in biofilm formation of Vibrio parahaemolyticus.Archives of microbiology · 2024Article
- High genetic diversity and different type VI secretion systems in Enterobacter species revealed by comparative genomics analysis.BMC microbiology · 2024Article
- Genetic Insights into Biofilm Formation by a Pathogenic Strain ofMicroorganisms · 2024Article
- Molecular mechanisms and environmental adaptations of flagellar loss and biofilm growth of Rhodanobacter under environmental stress.The ISME journal · 2024Article
- T6SS: A Key to Pseudomonas's Success in Biocontrol?Microorganisms · 2023Review
- Killing in the name of: T6SS structure and effector diversity.Microbiology (Reading, England) · 2023Review
- Article
- Global transcriptional analysis of Geobacter sulfurreducens gsu1771 mutant biofilm grown on two different support structures.PloS one · 2023Article
- From salty to thriving: plant growth promoting bacteria as nature's allies in overcoming salinity stress in plants.Frontiers in microbiology · 2023Review
- Transcriptomic Analysis of Vibrio parahaemolyticus Underlying the Wrinkly and Smooth Phenotypes.Microbiology spectrum · 2022Article
- Genome Wide Analysis of the Potato Soft Rot Pathogen Pectobacterium carotovorum Strain ICMP 5702 to Predict Novel Insights into Its Genetic Features.The plant pathology journal · 2022Article
- Metaphenotypes associated with recurrent genomic lineages ofFrontiers in microbiology · 2022Article
- Virulence Genotype and Correlation of Clinical Severeness with Presence of the Type VI Secretion System inInfection and drug resistance · 2022Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Type VI secretion systems (T6SSs) are widespread in Gram-negative bacteria, including Pseudomonas. These macromolecular machineries inject toxins directly into prokaryotic or eukaryotic prey cells. Hcp proteins are structural components of the extracellular part of this machinery. We recently reported that MFE01, an avirulent strain of Pseudomonas fluorescens, possesses at least two hcp genes, hcp1 and hcp2, encoding proteins playing important roles in interbacterial interactions. Indeed, P. fluorescens MFE01 can immobilise and kill diverse bacteria of various origins through the action of the Hcp1 or Hcp2 proteins of the T6SS. We show here that another Hcp protein, Hcp3, is involved in killing prey cells during co-culture on solid medium. Even after the mutation of hcp1, hcp2, or hcp3, MFE01 impaired biofilm formation by MFP05, a P. fluorescens strain isolated from human skin. These mutations did not reduce P. fluorescens MFE01 biofilm formation, but the three Hcp proteins were required for the completion of biofilm maturation. Moreover, a mutant with a disruption of one of the unique core component genes, MFE01ΔtssC, was unable to produce its own biofilm or inhibit MFP05 biofilm formation. Finally, MFE01 did not produce detectable N-acyl-homoserine lactones for quorum sensing, a phenomenon reported for many other P. fluorescens strains. Our results suggest a role for the T6SS in communication between bacterial cells, in this strain, under biofilm conditions.
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