ArticlePloS one2017
Proteomic Analysis of Kveim Reagent Identifies Targets of Cellular Immunity in Sarcoidosis.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 53 citations in OpenAlex.
- Sarcoidosis as an Autoimmune Disease.Frontiers in immunology · 2019Pooled it
- Article
- Sarcoidosis and Cancer: The Role of the Granulomatous Reaction as a Double-Edged Sword.Journal of clinical medicine · 2024Article
- Sarcoidosis and Autoimmune Inflammatory Syndrome Induced by Adjuvants.Life (Basel, Switzerland) · 2023Review
- The Overlap of Kidney Failure in Extrapulmonary Sarcoidosis in Children-Case Report and Review of Literature.International journal of molecular sciences · 2023Review
- Detection of Anti-Vimentin Antibodies in Patients with Sarcoidosis.Diagnostics (Basel, Switzerland) · 2022Article
- Systemic immune response to vimentin and granuloma formation in a model of pulmonary sarcoidosis.Journal of translational autoimmunity · 2022Article
- Sarcoidosis and autoimmunity: In the depth of a complex relationship.Frontiers in medicine · 2022Review
- Simultaneous testing of immunological sensitization to multiple antigens in sarcoidosis reveals an association with inorganic antigens specifically related to a fibrotic phenotype.Clinical and experimental immunology · 2021Article
- Inflammatory Pathways in Sarcoidosis.Advances in experimental medicine and biology · 2021Article
- Classical Examples of the Concept of the ASIA Syndrome.Biomolecules · 2020Review
- Management of Sarcoidosis, a Selection of Topical Items Updating.Journal of clinical medicine · 2020Article
- Key Players and Biomarkers of the Adaptive Immune System in the Pathogenesis of Sarcoidosis.International journal of molecular sciences · 2020Review
- Presence of concurrent sarcoid-like granulomas indicates better survival in cancer patients: a retrospective cohort study.ERJ open research · 2020Article
- Clinical Presentations, Pathogenesis, and Therapy of Sarcoidosis: State of the Art.Journal of clinical medicine · 2020Review
- Imbalance in B cell and T Follicular Helper Cell Subsets in Pulmonary Sarcoidosis.Scientific reports · 2020Article
- Environmental Risk Factors for Sarcoidosis.Frontiers in immunology · 2020Review
- The composition of the pulmonary microbiota in sarcoidosis - an observational study.Respiratory research · 2019Observational
- Article
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Authors and funding
13 authors at 5 institutions in 1 country.
Funding
Abstract
backgroundKveim-reagent (Kv) skin testing was a historical method of diagnosing sarcoidosis. Intradermal injection of treated sarcoidosis spleen tissue resulted in a granuloma response at injection site by 4-6 weeks. Previous work indicates proteins as the possible trigger of this reaction. We aimed to identify Kv-specific proteins and characterise the ex vivo response of Peripheral Blood Mononuclear Cells (PBMCs) from sarcoidosis, tuberculosis and healthy control patients when stimulated with both Kv and selected Kv-specific proteins.
methodsKv extracts were separated by 1D-SDS-PAGE and 2D-DIGE and then underwent mass spectrometric analysis for protein identification. Sarcoidosis and control PBMCs were first stimulated with Kv and then with three selected recombinant protein candidates which were identified from the proteomic analysis. PBMC secreted cytokines were subsequently measured by Multiplex Cytokine Assay.
resultsWe observed significantly increased IFN-γ and TNF-α secretion from Kv-stimulated PBMCs of sarcoidosis patients vs. PBMCs from healthy volunteers (IFN-γ: 207.2 pg/mL vs. 3.86 pg/mL, p = 0.0018; TNF-α: 2375 pg/mL vs. 42.82 pg/mL, p = 0.0003). Through proteomic approaches we then identified 74 sarcoidosis tissue-specific proteins. Of these, 3 proteins (vimentin, tubulin and alpha-actinin-4) were identified using both 1D-SDS-PAGE and 2D-DIGE. Data are available via ProteomeXchange with identifier PXD005150. Increased cytokine secretion was subsequently observed with vimentin stimulation of sarcoidosis PBMCs vs. tuberculosis PBMCs (IFN-γ: 396.6 pg/mL vs 0.1 pg/mL, p = 0.0009; TNF-α: 1139 pg/mL vs 0.1 pg/mL, p<0.0001). This finding was also observed in vimentin stimulation of sarcoidosis PBMCs compared to PBMCs from healthy controls (IFN-γ: 396.6 pg/mL vs. 0.1 pg/mL, p = 0.014; TNF-α: 1139 pg/mL vs 42.29 pg/mL, p = 0.027). No difference was found in cytokine secretion between sarcoidosis and control PBMCs when stimulated with either tubulin or alpha-actinin-4.
conclusionsStimulation with both Kveim reagent and vimentin induces a specific pro-inflammatory cytokine secretion from sarcoidosis PBMCs. Further investigation of cellular immune responses to Kveim-specific proteins may identify novel biomarkers to assist the diagnosis of sarcoidosis.
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