ArticleNature2017
MATRILINEAL, a sperm-specific phospholipase, triggers maize haploid induction.
Article in Nature, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 205 papers.
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Who cites it
205 citing papers in PubMed, 433 citations in OpenAlex.
- Advances and Applications of Genome Editing in Precision Crop Breeding.Plants (Basel, Switzerland) · 2026Review
- Pollen defective in guidance-1 (POD1) causes in vivo haploid induction in Brassica napus.The New phytologist · 2026Article
- Editing CsPOD7 triggers in vivo maternal haploid induction in cucumber.Journal of integrative plant biology · 2026Article
- Molecular understanding of plant pollination, fertilization, seed development and protoplast regeneration.Science China. Life sciences · 2026Review
- In vivo efficient maternal haploid production in melon via knockout of CmDMP genes.Plant physiology · 2026Article
- Article
- Diverse molecular mechanisms of in vivo haploid induction in plants: from fertilization interference to genome programming.Plant cell reports · 2026Review
- Genome-Wide Identification and Expression of the Mulberry PLA Family Under Drought and Salinity.Biology · 2026Article
- Basis of apomixis in flowering plants - state of the art and research perspectives.Plant reproduction · 2026Review
- Stepwise construction of the path to doubled haploid breeding in sorghum.Science advances · 2026Article
- Targeted CENH3 protein depletion in egg cells enables highly efficient haploid induction.Plant communications · 2026Article
- Rapid Genetic Stabilisation of Transgenic and Edited Wheat Through Transformation of Immature Haploid Embryos.Plant biotechnology journal · 2026Article
- Identification and Expression Analysis of the Goji Haploid-Inducible Gene DMP.International journal of molecular sciences · 2026Article
- QTL-Seq Identifies Extra QTLs and Candidate Genes Controlling High Haploid Induction Rate in Maize.Plants (Basel, Switzerland) · 2026Article
- Review
- Induction of Synthetic Apomixis in Two Sorghum Hybrids Enables Seed Yield and Genotype Preservation Over Multiple Generations.Plant biotechnology journal · 2026Article
- Next-generation CRISPR systems and advanced delivery paradigms for precision crop improvement: a comparative case study in sugar beet (Frontiers in plant science · 2026Review
- A two-stage screening approach integrated with GWAS for rare phenotypes such as spontaneous haploid genome doubling in maize.Frontiers in plant science · 2026Article
- In Vivo Rice Haploid-Induction System by an Egg Cell-Specific Peptidase Knockout.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Engineering Synthetic Apomixis in Hybrid Rice.Methods in molecular biology (Clifton, N.J.) · 2026Article
145 more citing papers are in PubMed but not listed here.
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sexual reproduction in flowering plants involves double fertilization, the union of two sperm from pollen with two sex cells in the female embryo sac. Modern plant breeders increasingly seek to circumvent this process to produce doubled haploid individuals, which derive from the chromosome-doubled cells of the haploid gametophyte. Doubled haploid production fixes recombinant haploid genomes in inbred lines, shaving years off the breeding process. Costly, genotype-dependent tissue culture methods are used in many crops, while seed-based in vivo doubled haploid systems are rare in nature and difficult to manage in breeding programmes. The multi-billion-dollar maize hybrid seed business, however, is supported by industrial doubled haploid pipelines using intraspecific crosses to in vivo haploid inducer males derived from Stock 6, first reported in 1959 (ref. 5), followed by colchicine treatment. Despite decades of use, the mode of action remains controversial. Here we establish, through fine mapping, genome sequencing, genetic complementation, and gene editing, that haploid induction in maize (Zea mays) is triggered by a frame-shift mutation in MATRILINEAL (MTL), a pollen-specific phospholipase, and that novel edits in MTL lead to a 6.7% haploid induction rate (the percentage of haploid progeny versus total progeny). Wild-type MTL protein localizes exclusively to sperm cytoplasm, and pollen RNA-sequence profiling identifies a suite of pollen-specific genes overexpressed during haploid induction, some of which may mediate the formation of haploid seed. These findings highlight the importance of male gamete cytoplasmic components to reproductive success and male genome transmittance. Given the conservation of MTL in the cereals, this discovery may enable development of in vivo haploid induction systems to accelerate breeding in crop plants.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.