ArticleNature nanotechnology2017
Single-molecule detection of protein efflux from microorganisms using fluorescent single-walled carbon nanotube sensor arrays.
Article in Nature nanotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
65 citing papers in PubMed, 221 citations in OpenAlex.
- Screening molecular recognition element-based SWCNT optical sensors for the inflammatory cytokine TNF-α.Biosensors & bioelectronics · 2026Article
- Optical Detection of Cellular Signals at Material Interfaces.Advanced healthcare materials · 2026Review
- High-Throughput SWCNT NIR-II Spectroscopy Enables Cell and In Vivo Applications.ACS nanoscience Au · 2026Article
- Chemical efflux imaging using an annular nanosensor array for in situ bladder cancer detection.Nature nanotechnology · 2026Article
- Rational Design of Optical Single-Walled Carbon Nanotube-Based Nanosensors with Biological Recognition Elements.Advanced sensor research · 2026Article
- Microenvironment-Guided Evolution of ssDNA-SWCNT Probes for Selective Recognition of Aggressive Prostate Cancer Phenotypes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- High-throughput SWCNT NIR-II screening enables cell andbioRxiv : the preprint server for biology · 2026Article
- Screening Molecular Recognition Element-Based SWCNT Optical Sensors for the Inflammatory Cytokine TNF-α.bioRxiv : the preprint server for biology · 2026Article
- Review
- Hetero-Functionalization of Carbon Nanotubes Termini with Single-Molecule Control.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- A salt-driven mechanism for precise chirality sorting of carbon nanotubes.Science advances · 2025Article
- Near-Infrared Fluorescent Single-Walled Carbon Nanotubes for Biosensing.Small (Weinheim an der Bergstrasse, Germany) · 2025Review
- Understanding DNA-encoded carbon nanotube sorting and sensing via sub-nm-resolution structural determination.Science advances · 2025Article
- Dynamic Tracking of Biological Processes Using Near-Infrared Fluorescent Single-Walled Carbon Nanotubes.ACS applied materials & interfaces · 2024Review
- Strategies, Challenges, and Prospects of Nanoparticles in Gynecological Malignancies.ACS omega · 2024Review
- Optimising CNT-FET biosensor design through modelling of biomolecular electrostatic gating and its application to β-lactamase detection.Nature communications · 2024Article
- Optical Aptamer-Based Cytokine Nanosensor Detects Macrophage Activation by Bacterial Toxins.ACS sensors · 2024Article
- Article
- Environmental damping and vibrational coupling of confined fluids within isolated carbon nanotubes.Nature communications · 2024Article
- Development and Evaluation of an Expedited System for Creation of Single Walled Carbon Nanotube Platforms.Carbon letters · 2024Article
5 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 4 institutions in 1 country.
Funding
Abstract
A distinct advantage of nanosensor arrays is their ability to achieve ultralow detection limits in solution by proximity placement to an analyte. Here, we demonstrate label-free detection of individual proteins from Escherichia coli (bacteria) and Pichia pastoris (yeast) immobilized in a microfluidic chamber, measuring protein efflux from single organisms in real time. The array is fabricated using non-covalent conjugation of an aptamer-anchor polynucleotide sequence to near-infrared emissive single-walled carbon nanotubes, using a variable chemical spacer shown to optimize sensor response. Unlabelled RAP1 GTPase and HIV integrase proteins were selectively detected from various cell lines, via large near-infrared fluorescent turn-on responses. We show that the process of E. coli induction, protein synthesis and protein export is highly stochastic, yielding variability in protein secretion, with E. coli cells undergoing division under starved conditions producing 66% fewer secreted protein products than their non-dividing counterparts. We further demonstrate the detection of a unique protein product resulting from T7 bacteriophage infection of E. coli, illustrating that nanosensor arrays can enable real-time, single-cell analysis of a broad range of protein products from various cell types.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.