ArticleScientific reports2017
Transit peptide elements mediate selective protein targeting to two different types of chloroplasts in the single-cell C4 species Bienertia sinuspersici.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 22 citations in OpenAlex.
- Molecular phylogenetics and evolutionary history ofFrontiers in plant science · 2026Review
- Expression and localization of two β-carbonic anhydrases inFrontiers in plant science · 2024Article
- Chloroplast proteostasis: A story of birth, life, and death.Plant communications · 2023Review
- Understanding protein import in diverse non-green plastids.Frontiers in genetics · 2023Review
- Comparative transcriptomics reveals the role of altered energy metabolism in the establishment of single-cell CFrontiers in plant science · 2023Article
- Article
- Development of C4 Biochemistry and Change in Expression of Markers for Photosystems I and II in the Single-Cell C4 Species, Bienertia sinuspersiciPlants (Basel, Switzerland) · 2022Article
- Biosynthetic approaches to efficient assimilation of COFrontiers in bioengineering and biotechnology · 2022Review
- Tissue-Specific Regulation of Plastid Protein Import via Transit-Peptide Motifs.The Plant cell · 2020Article
- Methods of analysis of chloroplast genomes of CPlant methods · 2020Article
- Electron Microscopy Views of Dimorphic Chloroplasts in C4 Plants.Frontiers in plant science · 2020Review
- Electron Tomography Analysis of Thylakoid Assembly and Fission in Chloroplasts of a Single-Cell C4 plant, Bienertia sinuspersici.Scientific reports · 2019Article
- A draft genome assembly of halophyte Suaeda aralocaspica, a plant that performs C4 photosynthesis within individual cells.GigaScience · 2019Article
- Agrobacterium-mediated transient transformation of Bienertia sinuspersici to assay recombinant protein distribution between dimorphic chloroplasts.Plant cell reports · 2019Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bienertia sinuspersici is a terrestrial plant that performs C4 photosynthesis within individual cells through operating a carbon concentrating mechanism between different subcellular domains including two types of chloroplasts. It is currently unknown how differentiation of two highly specialized chloroplasts within the same cell occurs as no similar cases have been reported. Here we show that this differentiation in photosynthetic cells of B. sinuspersici is enabled by a transit peptide (TP) mediated selective protein targeting mechanism. Mutations in the TPs cause loss of selectivity but not general loss of chloroplast import, indicating the mechanism operates by specifically blocking protein accumulation in one chloroplast type. Hybrid studies indicate that this selectivity is transferable to transit peptides of plants which perform C4 by cooperative function of chloroplasts between two photosynthetic cells. Codon swap experiments as well as introducing an artificial bait mRNA show that RNA affects are not crucial for the sorting process. In summary, our analysis shows how the mechanism of subcellular targeting to form two types of chloroplast within the same cell can be achieved. This information is not only crucial for understanding single-cell C4 photosynthesis; it provides new insights in control of subcellular protein targeting in cell biology.
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