ArticleJournal of biological rhythms2016
In-depth Characterization of Firefly Luciferase as a Reporter of Circadian Gene Expression in Mammalian Cells.
Article in Journal of biological rhythms, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 55 citations in OpenAlex.
- Advances in monitoring mammalian circadian components and their rhythms using reporter systems.RSC chemical biology · 2026Review
- Chemical biology approaches to study and target circadian clocks and their components.FEBS letters · 2026Review
- A cellular basis for the mammalian nocturnal-diurnal switch.Science (New York, N.Y.) · 2026Article
- Multiparametric Detection of Effects of TILs and Oncolytic Virotherapy on Xenograft Mouse Model of Glioblastoma.Biomedicines · 2025Article
- Dual-responsive synthetic gene circuit for dynamic biologic drug delivery via inflammatory and circadian signaling pathways.Journal of biological engineering · 2025Review
- Development of compounds for targeted degradation of mammalian cryptochrome proteins.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Article
- The Reindeer Circadian Clock Is Rhythmic and Temperature-compensated But Shows Evidence of Weak Coupling Between the Secondary and Core Molecular Clock Loops.Journal of biological rhythms · 2024Article
- Establishment of Translational Luciferase-Based Cancer Models to Evaluate Antitumoral Therapies.International journal of molecular sciences · 2024Article
- Computational modeling establishes mechanotransduction as a potent modulator of the mammalian circadian clock.Journal of cell science · 2024Article
- Quantitative analysis of electroporation-mediated intracellular delivery via bioorthogonal luminescent reaction.Communications chemistry · 2024Article
- Bmal1 integrates circadian function and temperature sensing in the suprachiasmatic nucleus.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- A Comprehensive Exploration of Bioluminescence Systems, Mechanisms, and Advanced Assays for Versatile Applications.Biochemistry research international · 2024Review
- Magnetic Ion Channel Activation (MICA)-Enabled Screening Assay: A Dynamic Platform for Remote Activation of Mechanosensitive Ion Channels.International journal of molecular sciences · 2023Article
- Imaging Techniques in Pharmacological Precision Medicine.Handbook of experimental pharmacology · 2023Article
- Compensatory ion transport buffers daily protein rhythms to regulate osmotic balance and cellular physiology.Nature communications · 2021Article
- The development and kinetics of functional antibody-dependent cell-mediated cytotoxicity (ADCC) to SARS-CoV-2 spike protein.Virology · 2021Article
- Stochastic light concentration from 3D to 2D reveals ultraweak chemi- and bioluminescence.Scientific reports · 2021Article
- Targeted modification of the Per2 clock gene alters circadian function in mPer2luciferase (mPer2Luc) mice.PLoS computational biology · 2021Article
- CRYPTOCHROMES confer robustness, not rhythmicity, to circadian timekeeping.The EMBO journal · 2021Article
- Bioluminescent Sensors for CaFrontiers in bioengineering and biotechnology · 2021Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Firefly luciferase (Fluc) is frequently used to report circadian gene expression rhythms in mammalian cells and tissues. During longitudinal assays it is generally assumed that enzymatic substrates are in saturating excess, such that total bioluminescence is directly proportional to Fluc protein level. To test this assumption, we compared the enzyme kinetics of purified luciferase with its activity in mammalian cells. We found that Fluc activity in solution has a lower Michaelis constant (K
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.