ArticleFrontiers in cellular and infection microbiology2016
Flow Cytometry Sorting to Separate Viable Giant Viruses from Amoeba Co-culture Supernatants.
Article in Frontiers in cellular and infection microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- Evaluating the utility of a nanoscale flow cytometer for detection of surface proteins on HIV and extracellular vesicles.Virology journal · 2026Article
- Usurpativirus massiliensis, a new giant virus related to clandestinovirus.Virology journal · 2025Article
- Flow virometry: recent advancements, best practices, and future frontiers.Journal of virology · 2025Review
- Unexpected diversity and ecological significance of uncultivable large virus-like particles in aquatic environments.ISME communications · 2025Article
- Asfarviruses and Closely Related Giant Viruses.Viruses · 2023Review
- Article
- Flow virometry for process monitoring of live virus vaccines-lessons learned from ERVEBO.Scientific reports · 2021Article
- Flow Virometry Quantification of Host Proteins on the Surface of HIV-1 Pseudovirus Particles.Viruses · 2020Article
- A Two-Dimensional Affinity Capture and Separation Mini-Platform for the Isolation, Enrichment, and Quantification of Biomarkers and Its Potential Use for Liquid Biopsy.Biomedicines · 2020Review
- Article
- Single Cell Metabolomics: A Future Tool to Unmask Cellular Heterogeneity and Virus-Host Interaction in Context of Emerging Viral Diseases.Frontiers in microbiology · 2020Review
- Virophages of Giant Viruses: An Update at Eleven.Viruses · 2019Review
- Review
- High-throughput isolation of giant viruses using high-content screening.Communications biology · 2019Article
- Correlative light electron microscopy of giant viruses with the SECOM system.New microbes and new infections · 2018Article
- Flow virometry as a tool to study viruses.Methods (San Diego, Calif.) · 2018Review
- Flow Virometry: a Powerful Tool To Functionally Characterize Viruses.Journal of virology · 2018Review
- A Large Open Pangenome and a Small Core Genome for Giant Pandoraviruses.Frontiers in microbiology · 2018Article
- Pacmanvirus, a New Giant Icosahedral Virus at the Crossroads between Asfarviridae and Faustoviruses.Journal of virology · 2017Article
- A Student's Guide to Giant Viruses Infecting Small Eukaryotes: From Acanthamoeba to Zooxanthellae.Viruses · 2017Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Flow cytometry has contributed to virology but has faced many drawbacks concerning detection limits, due to the small size of viral particles. Nonetheless, giant viruses changed many concepts in the world of viruses, as a result of their size and hence opened up the possibility of using flow cytometry to study them. Recently, we developed a high throughput isolation of viruses using flow cytometry and protozoa co-culture. Consequently, isolating a viral mixture in the same sample became more common. Nevertheless, when one virus multiplies faster than others in the mixture, it is impossible to obtain a pure culture of the minority population. Here, we describe a robust sorting system, which can separate viable giant virus mixtures from supernatants. We tested three flow cytometry sorters by sorting artificial mixtures. Purity control was assessed by electron microscopy and molecular biology. As proof of concept, we applied the sorting system to a co-culture supernatant taken from a sample containing a viral mixture that we couldn't separate using end point dilution. In addition to isolating the quick-growing
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