ArticleAsian-Australasian journal of animal sciences2017
Functional analysis of SH3 domain containing ring finger 2 during the myogenic differentiation of quail myoblast cells.
Article in Asian-Australasian journal of animal sciences, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- - Invited Review - Gene-editing techniques and their applications in livestock and beyond.Animal bioscience · 2023Article
- Functional analyses of miRNA-146b-5p during myogenic proliferation and differentiation in chicken myoblasts.BMC molecular and cell biology · 2020Article
- Muscle differentiation induced up-regulation of calcium-related gene expression in quail myoblasts.Asian-Australasian journal of animal sciences · 2018Article
- Molecular characterization of the apoptosis-related SH3RF1 and SH3RF2 genes and their association with exercise performance in Arabian horses.BMC veterinary research · 2018Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveOwing to the public availability of complete genome sequences, including avian species, massive bioinformatics analyses may be conducted for computational gene prediction and the identification of gene regulatory networks through various informatics tools. However, to evaluate the biofunctional activity of a predicted target gene,
methodsDue to a lack of quail genomic sequence information, we first identified the partial genomic structure and sequences of the quail SH3 domain containing ring finger 2 (
resultsThrough a T7 endonuclease I assay and genotyping analysis, we established an SH3RF2 knockout (KO) QM7#4 subline with 61 and 155 nucleotide deletion mutations in
conclusionWe did not detect any statistically significant role of SH3RF2 during myotube differentiation in QM7 myoblast cells. However, additional experiments are necessary to examine the biofunctional activity of SH3RF2 in cell proliferation and muscle growth.
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