ArticleMetabolic engineering2017
Metabolic engineering of Clostridium autoethanogenum for selective alcohol production.
Article in Metabolic engineering, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
70 citing papers in PubMed, 244 citations in OpenAlex.
- Overexpression of endogenous aldehyde:ferredoxin oxidoreductase genes enhances ethanol biosynthesis in Clostridium autoethanogenum.World journal of microbiology & biotechnology · 2026Article
- Mixing regimes shape microbial community composition, nutrient regimes, and plant growth attributes in Jeevamrit: metagenomics and culturomics-based insights.Scientific reports · 2026Article
- Elucidating the roles of essential genes in autotrophic metabolism and cell morphology of Clostridium ljungdahlii by CRISPRi.Applied microbiology and biotechnology · 2026Article
- Carbon-monoxide-driven bioethanol production operates through a tungsten-dependent catalyst.Nature chemical biology · 2026Article
- Synthetic Microbial Cocultivation for Targeted Production of Odd-Chain Carboxylates and Alcohols from Carbon Monoxide.Environmental science & technology · 2025Article
- Methanothermobacter thermautotrophicus and Alternative Methanogens: Archaea-Based Production.Advances in biochemical engineering/biotechnology · 2025Review
- Understanding microbial syngas fermentation rates.Applied microbiology and biotechnology · 2024Review
- Influence of Hydrogen and Ethanol Addition in Methanogen-Free Mixed Culture Syngas Fermentations in Trickle Bed Reactors.Molecules (Basel, Switzerland) · 2024Article
- Harnessing acetogenic bacteria for one-carbon valorization toward sustainable chemical production.RSC chemical biology · 2024Review
- Isolation and characterization of novel acetogenic Moorella strains for employment as potential thermophilic biocatalysts.FEMS microbiology ecology · 2024Article
- Impact of cathodic pH and bioaugmentation on acetate and CHRSC advances · 2024Article
- Redirecting electron flow in Acetobacterium woodii enables growth on CO and improves growth on formate.Nature communications · 2024Article
- Adaptive laboratory evolution of Clostridium autoethanogenum to metabolize COMicrobial biotechnology · 2024Article
- Recent progress in engineeringSynthetic and systems biotechnology · 2024Review
- Probing efficient microbial COMicrobial biotechnology · 2024Article
- A Versatile Aldehyde: Ferredoxin Oxidoreductase from the Organic Acid ReducingInternational journal of molecular sciences · 2024Article
- Evaluating the feasibility of medium-chain oleochemical synthesis using microbial chain elongation.Journal of industrial microbiology & biotechnology · 2024Article
- Engineered acetogenic bacteria as microbial cell factory for diversified biochemicals.Frontiers in bioengineering and biotechnology · 2024Review
- The potential of native and engineered Clostridia for biomass biorefining.Frontiers in bioengineering and biotechnology · 2024Review
- The role of ethanol oxidation during carboxydotrophic growth of Clostridium autoethanogenum.Microbial biotechnology · 2023Article
10 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Gas fermentation using acetogenic bacteria such as Clostridium autoethanogenum offers an attractive route for production of fuel ethanol from industrial waste gases. Acetate reduction to acetaldehyde and further to ethanol via an aldehyde: ferredoxin oxidoreductase (AOR) and alcohol dehydrogenase has been postulated alongside the classic pathway of ethanol formation via a bi-functional aldehyde/alcohol dehydrogenase (AdhE). Here we demonstrate that AOR is critical to ethanol formation in acetogens and inactivation of AdhE led to consistently enhanced autotrophic ethanol production (up to 180%). Using ClosTron and allelic exchange mutagenesis, which was demonstrated for the first time in an acetogen, we generated single mutants as well as double mutants for both aor and adhE isoforms to confirm the role of each gene. The aor1+2 double knockout strain lost the ability to convert exogenous acetate, propionate and butyrate into the corresponding alcohols, further highlighting the role of these enzymes in catalyzing the thermodynamically unfavourable reduction of carboxylic acids into alcohols.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.