Evidence map›Paper›PMID 28110145›Full record

ArticleVirology2017

VirusSeeker, a computational pipeline for virus discovery and virome composition analysis.

Guoyan Zhao, Guang Wu, Efrem S Lim, Lindsay Droit, Siddharth Krishnamurthy, Dan H Barouch, Herbert W Virgin, David Wang

Abstract read
In one paragraph

Article in Virology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.

0numbers the graph read from it
0cells of the map it votes in
85citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

85 citing papers in PubMed.

  1. Article
  2. Ethno-etiology meets virology: land leeches (family: Haemadipsidae) as potential disease vectors.Transactions of the Royal Society of Tropical Medicine and Hygiene · 2026
    Review
  3. Article
  4. Article
  5. Gut virome dynamics: from commensal to critical player in health and disease.Nature reviews. Gastroenterology & hepatology · 2026
    Review
  6. Review
  7. Article
  8. Article
  9. A review of neural networks for metagenomic binning.Briefings in bioinformatics · 2025
    Review
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Review
  16. Article
  17. Review
  18. Article
  19. Article
  20. Article

25 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Guoyan ZhaoDepartments of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO, USA. Electronic address: gzhao@wustl.edu.
Guang WuDepartment of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO, USA.
Efrem S LimDepartment of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO, USA.
Lindsay DroitDepartments of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO, USA.
Siddharth KrishnamurthyDepartment of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO, USA.
Dan H BarouchCenter for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA; Ragon Institute of MGH, MIT, and Harvard, Boston, MA 02114, USA.
Herbert W VirginDepartments of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO, USA.
David WangDepartments of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO, USA; Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO, USA. Electronic address: davewang@wustl.edu.

Funding

Virology and Smallpox VaccinationsU54AI057160 · NIAID · WASHINGTON UNIVERSITY · PI RAY, RANJIT · 2003 to 2013
$80.9M
Genetic and Pathway Analysis CoreU19AI109725 · NIAID · WASHINGTON UNIVERSITY · PI VIRGIN, HERBERT W · 2014 to 2018
$32.7M
THE VIROME AND MICROBIOME IN HEALTH AND BK DISEASE OF KIDNEY TRANSPLANT PATIENTSK99DK107923 · NIDDK · WASHINGTON UNIVERSITY · PI LIM, EFREM · 2016 to 2016
$92k
NIAID NIH HHS U19 AI109725NIAID NIH HHS U54 AI057160NIDDK NIH HHS K99 DK107923
6 · The paper itself

Abstract

The advent of Next Generation Sequencing (NGS) has vastly increased our ability to discover novel viruses and to systematically define the spectrum of viruses present in a given specimen. Such studies have led to the discovery of novel viral pathogens as well as broader associations of the virome with diverse diseases including inflammatory bowel disease, severe acute malnutrition and HIV/AIDS. Critical to the success of these efforts are robust bioinformatic pipelines for rapid classification of microbial sequences. Existing computational tools are typically focused on either eukaryotic virus discovery or virome composition analysis but not both. Here we present VirusSeeker, a BLAST-based NGS data analysis pipeline designed for both purposes. VirusSeeker has been successfully applied in several previously published virome studies. Here we demonstrate the functionality of VirusSeeker in both novel virus discovery and virome composition analysis.

Indexed as

AnimalsComputational BiologyDNA, ViralFecesGenome, ViralHigh-Throughput Nucleotide SequencingMacaca mulattaMetagenomicsMonkey DiseasesSequence Analysis, DNAVirusesDNA, ViralNovel virusPathogen discoveryViral metagenomicsViromeVirusSeeker

Identifiers

PMID28110145
PMCPMC5326578

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.