ArticleProtein expression and purification2017
Heterologous expression and characterization of plant Taxadiene-5α-Hydroxylase (CYP725A4) in Escherichia coli.
Article in Protein expression and purification, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 25 citations in OpenAlex.
- Inspired by taxol: biosynthesis and engineering of hypericin and other phytochemicals in Hypericum.Planta · 2026Review
- Recent advances in paclitaxel biosynthesis and regulation.Journal of experimental botany · 2025Review
- Computational redesign of taxane-10β-hydroxylase for de novo biosynthesis of a key paclitaxel intermediate.Applied microbiology and biotechnology · 2023Article
- Way to efficient microbial paclitaxel mass production.Synthetic and systems biotechnology · 2023Review
- Hydroxylases involved in terpenoid biosynthesis: a review.Bioresources and bioprocessing · 2023Review
- Challenges in the Heterologous Production of Furanocoumarins inMolecules (Basel, Switzerland) · 2022Article
- Exploring optimal Taxol® CYP725A4 activity in Saccharomyces cerevisiae.Microbial cell factories · 2022Article
- Construction of an Escherichia coli cell factory to synthesize taxadien-5α-ol, the key precursor of anti-cancer drug paclitaxel.Bioresources and bioprocessing · 2022Article
- Recent Advances in Metabolic Engineering, Protein Engineering, and Transcriptome-Guided Insights Toward Synthetic Production of Taxol.Frontiers in bioengineering and biotechnology · 2021Review
- Redesign and reconstruction of a steviol-biosynthetic pathway for enhanced production of steviol in Escherichia coli.Microbial cell factories · 2020Article
- Gene isolation, heterologous expression, purification and functional confirmation of sesamin synthase fromBiotechnology reports (Amsterdam, Netherlands) · 2019Article
- Engineering the unicellular alga Phaeodactylum tricornutum for high-value plant triterpenoid production.Plant biotechnology journal · 2019Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
Taxadiene-5α-Hydroxylase (CYP725A4) is a membrane-bound plant cytochrome P450 that catalyzes the oxidation of taxadiene to taxadiene-5α-ol. This oxidation is a key step in the production of the valuable cancer therapeutic and natural plant product, taxol. In this work, we report the bacterial expression and purification of six different constructs of CYP725A4. All six of these constructs are N-terminally modified and three of them are fused to cytochrome P450 reductase to form a chimera construct. The construct with the highest yield of CYP725A4 protein was then selected for substrate binding and kinetic analysis. Taxadiene binding followed type-1 substrate patterns with an observed K
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Registered trials
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