Evidence map›Paper›PMID 28109445›Full record

ArticleMethods in enzymology2017

Identification of Novel Macropinocytosing Human Antibodies by Phage Display and High-Content Analysis.

K D Ha, S M Bidlingmaier, Y Su, N-K Lee, B Liu

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in enzymology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
2.4field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Review
  3. Macropinocytosis: mechanism and targeted therapy in cancers.American journal of cancer research · 2021
    Review
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

K D HaUCSF Helen Diller Family Comprehensive Cancer Center, University of California at San Francisco, San Francisco, CA, United States.
S M BidlingmaierUCSF Helen Diller Family Comprehensive Cancer Center, University of California at San Francisco, San Francisco, CA, United States.
Y SuUCSF Helen Diller Family Comprehensive Cancer Center, University of California at San Francisco, San Francisco, CA, United States.
N-K LeeUCSF Helen Diller Family Comprehensive Cancer Center, University of California at San Francisco, San Francisco, CA, United States.
B LiuUCSF Helen Diller Family Comprehensive Cancer Center, University of California at San Francisco, San Francisco, CA, United States. Electronic address: bin.liu@ucsf.edu.
UCSF Helen Diller Family Comprehensive Cancer Center · US

Funding

Mapping a clinically significant internalizing tumor epitope space.R01CA118919 · NCI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI LIU, BIN · 2006 to 2013
$2.5M
Internalizing human antibody-targeted nanosized siRNA therapeuticsR01CA171315 · NCI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI LIU, BIN · 2012 to 2016
$1.6M
Identifying antigens bound by novel scFvs targeting all subtypes of mesotheliomaR01CA129491 · NCI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI LIU, BIN · 2010 to 2013
$1.2M
NCI NIH HHS R01 CA118919NCI NIH HHS R01 CA129491NCI NIH HHS R01 CA171315
6 · The paper itself

Abstract

Internalizing antibodies have great potential for the development of targeted therapeutics. Antibodies that internalize via the macropinocytosis pathway are particularly promising since macropinocytosis is capable of mediating rapid, bulk uptake and is selectively upregulated in many cancers. We hereby describe a method for identifying antibodies that internalize via macropinocytosis by screening phage-displayed single-chain antibody selection outputs with an automated fluorescent microscopy-based high-content analysis platform. Furthermore, this method can be similarly applied to other endocytic pathways if other fluorescent, pathway-specific, soluble markers are available.

Indexed as

AntibodiesAntibody AffinityAntibody SpecificityAntigens, NeoplasmEndocytosisHumansNeoplasmsPeptide LibraryAntibodiesAntigens, NeoplasmPeptide LibraryCancer cell membrane dynamicsCancer metabolismEndocytosis pathwayHigh-content analysisHigh-throughput screeningHuman monoclonal antibodiesMacropinocytosisPhage antibody display

Identifiers

PMID28109445
PMCPMC8671048
OpenAlexW2558133614

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.