ArticleMethods in enzymology2017
Analysis of the Proteome of Hair-Cell Stereocilia by Mass Spectrometry.
Article in Methods in enzymology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 8 citations in OpenAlex.
- Protrusion growth driven by myosin-generated force.Developmental cell · 2023Article
- Espin overexpression causes stereocilia defects and provides an anti-capping effect on actin polymerization.Cytoskeleton (Hoboken, N.J.) · 2022Article
- Semicircular canal biomechanics in health and disease.Journal of neurophysiology · 2019Review
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Characterization of proteins that mediate mechanotransduction by hair cells, the sensory cells of the inner ear, is hampered by the scarcity of these cells and their sensory organelle, the hair bundle. Mass spectrometry, with its high sensitivity and identification precision, is the ideal method for determining which proteins are present in bundles and what proteins they interact with. We describe here the isolation of mouse hair bundles, as well as preparation of bundle protein samples for mass spectrometry. We also describe protocols for data-dependent (shotgun) and parallel reaction monitoring (targeted) mass spectrometry that allow us to identify and quantify proteins of the hair bundle. These sensitive methods are particularly useful for comparing proteomes of wild-type mice and mice with deafness mutations affecting hair-bundle proteins.
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