Evidence map›Paper›PMID 28108553›Full record

ArticleG3 (Bethesda, Md.)2017

The Bright Fluorescent Protein mNeonGreen Facilitates Protein Expression Analysis

Lola Hostettler, Laura Grundy, Stéphanie Käser-Pébernard, Chantal Wicky, William R Schafer, Dominique A Glauser

Open access · goldAbstract read
In one paragraph

Article in G3 (Bethesda, Md.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.

0numbers the graph read from it
0cells of the map it votes in
57citing papers in PubMed
3.9field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

57 citing papers in PubMed, 87 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Efficient and Stable Subcellular Protein Labeling inPathogens (Basel, Switzerland) · 2026
    Article
  5. Identification of optimal fluorophores for use in theMolecular biology of the cell · 2026
    Article
  6. Article
  7. Review
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Expanding the genetic toolbox for the obligate human pathogenFrontiers in bioengineering and biotechnology · 2024
    Article
  19. mNeonGreen aggregates when overexpressed inmicroPublication biology · 2024
    Article
  20. Flavivirus Concentrates Host ER in Main Replication Compartments to Facilitate Replication.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Lola HostettlerDepartment of Biology, University of Fribourg, 1700 Fribourg, Switzerland.
Laura GrundyNeurobiology Division, MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK.
Stéphanie Käser-PébernardDepartment of Biology, University of Fribourg, 1700 Fribourg, Switzerland.
Chantal WickyDepartment of Biology, University of Fribourg, 1700 Fribourg, Switzerland.
William R SchaferNeurobiology Division, MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK.
Dominique A GlauserDepartment of Biology, University of Fribourg, 1700 Fribourg, Switzerland dominique.glauser@unifr.ch.
University of Fribourg · CHMRC Laboratory of Molecular Biology · GB

Funding

Medical Research Council MC_U105185857Wellcome Trust WT10378MA
6 · The paper itself

Abstract

The Green Fluorescent Protein (GFP) has been tremendously useful in investigating cell architecture, protein localization, and protein function. Recent developments in transgenesis and genome editing methods now enable working with fewer transgene copies and, consequently, with physiological expression levels. However, lower signal intensity might become a limiting factor. The recently developed mNeonGreen protein is a brighter alternative to GFP

Indexed as

AnimalsCaenorhabditis elegansCell TrackingGreen Fluorescent ProteinsIntestinesLuminescent ProteinsMicroscopy, FluorescenceNeuronsOptical ImagingTransgenesGreen Fluorescent ProteinsLuminescent Proteinsbright fluorescent proteinexpression reporterin vivo imagingnematodeworm

Identifiers

PMID28108553
PMCPMC5295605
OpenAlexW2574960833

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.