ArticlePLoS pathogens2017
RNAi-Based Functional Genomics Identifies New Virulence Determinants in Mucormycosis.
Article in PLoS pathogens, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers, 1 of them a synthesis that pooled it.
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Who cites it
46 citing papers in PubMed, 1 synthesis or guideline pooled it, 74 citations in OpenAlex.
- Epidemiology and Pathophysiology of COVID-19-Associated Mucormycosis: India Versus the Rest of the World.Mycopathologia · 2021Pooled it
- Domain-Targeted RNAi of VeA Reveals Its Essential Role in the Fusarium oxysporum-Pseudostellaria heterophylla Interaction.Molecular plant pathology · 2026Article
- Light restores sporulation in Rhizopus microsporus cured of its endosymbionts, unveiling their role in fitness and virulence.The ISME journal · 2026Article
- Understanding the biology of mucormycosis using contemporary omics tools: a review.Biotechnologia · 2026Review
- Mucormycosis and COVID-19: Unraveling the Interplay of Fungal Infection in a Global Health Crisis: An Overview.Infectious disorders drug targets · 2025Review
- Role of the putativeMicrobial cell (Graz, Austria) · 2025Article
- Myo5B plays a significant role in the hyphal growth and virulence of the human pathogenic fungusMicrobiology (Reading, England) · 2024Article
- Mucormycosis: A Rare disease to Notifiable Disease.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Review
- The RNAi Machinery in the FungusNon-coding RNA · 2024Article
- Molecular mechanisms that govern infection and antifungal resistance in Mucorales.Microbiology and molecular biology reviews : MMBR · 2024Review
- AlternativeFrontiers in cellular and infection microbiology · 2024Review
- Comparative genomics predict specific genes in potential mucorales identification.Archives of microbiology · 2023Article
- Characterization of the Sterol 24-C-Methyltransferase Genes Reveals a Network of Alternative Sterol Biosynthetic Pathways inMicrobiology spectrum · 2023Article
- Review
- Mucormycosis in 2023: an update on pathogenesis and management.Frontiers in cellular and infection microbiology · 2023Review
- The emergence of COVID-19 associated mucormycosis: a review of cases from 18 countries.The Lancet. Microbe · 2022Review
- Secretion of the siderophore rhizoferrin is regulated by the cAMP-PKA pathway and is involved in the virulence of Mucor lusitanicus.Scientific reports · 2022Article
- Genetic Manipulation in Mucorales and New Developments to Study Mucormycosis.International journal of molecular sciences · 2022Review
- Myosin-II proteins are involved in the growth, morphogenesis, and virulence of the human pathogenic fungusFrontiers in cellular and infection microbiology · 2022Article
- Stable and reproducible homologous recombination enables CRISPR-based engineering in the fungusCell reports methods · 2021Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mucorales are an emerging group of human pathogens that are responsible for the lethal disease mucormycosis. Unfortunately, functional studies on the genetic factors behind the virulence of these organisms are hampered by their limited genetic tractability, since they are reluctant to classical genetic tools like transposable elements or gene mapping. Here, we describe an RNAi-based functional genomic platform that allows the identification of new virulence factors through a forward genetic approach firstly described in Mucorales. This platform contains a whole-genome collection of Mucor circinelloides silenced transformants that presented a broad assortment of phenotypes related to the main physiological processes in fungi, including virulence, hyphae morphology, mycelial and yeast growth, carotenogenesis and asexual sporulation. Selection of transformants with reduced virulence allowed the identification of mcplD, which encodes a Phospholipase D, and mcmyo5, encoding a probably essential cargo transporter of the Myosin V family, as required for a fully virulent phenotype of M. circinelloides. Knock-out mutants for those genes showed reduced virulence in both Galleria mellonella and Mus musculus models, probably due to a delayed germination and polarized growth within macrophages. This study provides a robust approach to study virulence in Mucorales and as a proof of concept identified new virulence determinants in M. circinelloides that could represent promising targets for future antifungal therapies.
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