ArticlePloS one2017
Deletion of pH Regulator pac-3 Affects Cellulase and Xylanase Activity during Sugarcane Bagasse Degradation by Neurospora crassa.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 25 citations in OpenAlex.
- Cumulative expression of heterologous XlnR regulatory modules and AraRBiotechnology for biofuels and bioproducts · 2024Article
- Intracellular Nitric Oxide and cAMP Are Involved in Cellulolytic Enzyme Production inInternational journal of molecular sciences · 2023Article
- Plasma Promotes Fungal Cellulase Production by Regulating the Levels of Intracellular NO and CaInternational journal of molecular sciences · 2022Article
- Insights into the Lignocellulose-Degrading Enzyme System ofMicrobiology spectrum · 2021Article
- Analysis of the phosphorylome of trichoderma reesei cultivated on sugarcane bagasse suggests post-translational regulation of the secreted glycosyl hydrolase Cel7A.Biotechnology reports (Amsterdam, Netherlands) · 2021Article
- Proteomic analysis reflects an environmental alkalinization-coupled pH-dependent mechanism of regulating lignocellulases inBiotechnology for biofuels · 2020Article
- High QualityFrontiers in bioengineering and biotechnology · 2020Article
- The pH Signaling Transcription Factor PAC-3 Regulates Metabolic and Developmental Processes in Pathogenic Fungi.Frontiers in microbiology · 2019Article
- Extracellular vesicles carry cellulases in the industrial fungusBiotechnology for biofuels · 2019Article
- Functional diversity in the pH signaling pathway: an overview of the pathway regulation in Neurospora crassa.Current genetics · 2018Review
- mus-52 disruption and metabolic regulation in Neurospora crassa: Transcriptional responses to extracellular phosphate availability.PloS one · 2018Article
- Conservation and diversity of the regulators of cellulolytic enzyme genes in Ascomycete fungi.Current genetics · 2017Review
- Regulators of plant biomass degradation in ascomycetous fungi.Biotechnology for biofuels · 2017Review
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microorganisms play a vital role in bioethanol production whose usage as fuel energy is increasing worldwide. The filamentous fungus Neurospora crassa synthesize and secrete the major enzymes involved in plant cell wall deconstruction. The production of cellulases and hemicellulases is known to be affected by the environmental pH; however, the regulatory mechanisms of this process are still poorly understood. In this study, we investigated the role of the pH regulator PAC-3 in N. crassa during their growth on sugarcane bagasse at different pH conditions. Our data indicate that secretion of cellulolytic enzymes is reduced in the mutant Δpac-3 at alkaline pH, whereas xylanases are positively regulated by PAC-3 in acidic (pH 5.0), neutral (pH 7.0), and alkaline (pH 10.0) medium. Gene expression profiles, evaluated by real-time qPCR, revealed that genes encoding cellulases and hemicellulases are also subject to PAC-3 control. Moreover, deletion of pac-3 affects the expression of transcription factor-encoding genes. Together, the results suggest that the regulation of holocellulase genes by PAC-3 can occur as directly as in indirect manner. Our study helps improve the understanding of holocellulolytic performance in response to PAC-3 and should thereby contribute to the better use of N. crassa in the biotechnology industry.
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