ArticleJournal of the American Chemical Society2017
Orthogonal Luciferase-Luciferin Pairs for Bioluminescence Imaging.
Article in Journal of the American Chemical Society, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed.
- Recent Proceedings of Advanced Electrochemiluminescence Imaging Technology and Applications in Accurate Analysis.Chemical & biomedical imaging · 2026Review
- Continuous Hypermutation and Evolution of Luciferase Variants.ACS chemical biology · 2026Article
- Protein engineering: status report.Protein engineering, design & selection : PEDS · 2026Review
- Ultrasensitive and long-lasting bioluminescence immunoassay for point-of-care viral antigen detection.Nature biomedical engineering · 2025Article
- A Comprehensive Review: Versatile Imaging Probe Based on Chemical Materials for Biomedical Applications.Applied biochemistry and biotechnology · 2025Review
- PEGylated ATP-Independent Luciferins for Noninvasive High-Sensitivity High-Speed Bioluminescence Imaging.ACS chemical biology · 2025Article
- Bright Red Bioluminescence from Semisynthetic NanoLuc (sNLuc).ACS chemical biology · 2024Article
- Medicinal Chemistry and NMR Driven Discovery of Novel UDP-glucuronosyltransferase 1A Inhibitors That Overcome Therapeutic Resistance in Cells.Journal of molecular biology · 2024Article
- The Application of Bio-orthogonality for In Vivo Animal Imaging.Chemical & biomedical imaging · 2023Review
- Recent Applications and Future Perspectives of Chemiluminescent and Bioluminescent Imaging Technologies.Chemical & biomedical imaging · 2023Review
- Biochemical Analysis Leads to Improved Orthogonal Bioluminescent Tools.Chembiochem : a European journal of chemical biology · 2023Article
- Red-Shifted Coumarin Luciferins for Improved Bioluminescence Imaging.Journal of the American Chemical Society · 2023Article
- Synthesis of 2-Cyanobenzothiazoles via Pd-Catalyzed/Cu-Assisted C-H Functionalization/Intramolecular C-S Bond Formation fromMolecules (Basel, Switzerland) · 2022Article
- Multiplexed bioluminescence imaging with a substrate unmixing platform.Cell chemical biology · 2022Article
- Computational Investigation of Substituent Effects on the Fluorescence Wavelengths of Oxyluciferin Analogs.Journal of photochemistry and photobiology. A, Chemistry · 2022Article
- Multiplexed bioluminescence microscopy via phasor analysis.Nature methods · 2022Article
- Iodine-DMSO mediated conversion ofRSC advances · 2022Article
- Design, Synthesis, and Evaluation of Near-Infrared Fluorescent Molecules Based on 4H-1-Benzopyran Core.Molecules (Basel, Switzerland) · 2021Article
- Coumarin luciferins and mutant luciferases for robust multi-component bioluminescence imaging.Chemical science · 2021Article
- Multicomponent Bioluminescence Imaging with Naphthylamino Luciferins.Chembiochem : a European journal of chemical biology · 2021Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Bioluminescence imaging with luciferase-luciferin pairs is widely used in biomedical research. Several luciferases have been identified in nature, and many have been adapted for tracking cells in whole animals. Unfortunately, the optimal luciferases for imaging in vivo utilize the same substrate and therefore cannot easily differentiate multiple cell types in a single subject. To develop a broader set of distinguishable probes, we crafted custom luciferins that can be selectively processed by engineered luciferases. Libraries of mutant enzymes were iteratively screened with sterically modified luciferins, and orthogonal enzyme-substrate "hits" were identified. These tools produced light when complementary enzyme-substrate partners interacted both in vitro and in cultured cell models. Based on their selectivity, these designer pairs will bolster multicomponent imaging and enable the direct interrogation of cell networks not currently possible with existing tools. Our screening platform is also general and will expedite the identification of more unique luciferases and luciferins, further expanding the bioluminescence toolkit.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.