ArticleBMC plant biology2016
Gene expression profiling of flax (Linum usitatissimum L.) under edaphic stress.
Article in BMC plant biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 56 citations in OpenAlex.
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- Transcriptome map and genome annotation of flax line 3896.Frontiers in plant science · 2025Article
- Expression ofPlants (Basel, Switzerland) · 2024Article
- KeyInternational journal of molecular sciences · 2023Article
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- Bioinformatics Analysis of WRKY Family Genes in Flax (Life (Basel, Switzerland) · 2023Article
- Development and Complex Application of Methods for the Identification of Mutations in thePlants (Basel, Switzerland) · 2022Article
- Transposable Elements in the Revealing of Polymorphism-Based Differences in the Seeds of Flax Varieties Grown in Remediated Chernobyl Area.Plants (Basel, Switzerland) · 2022Article
- Spatiotemporal Transcriptomic Atlas of Developing Embryos and Vegetative Tissues in Flax.Plants (Basel, Switzerland) · 2022Article
- Discovery and functional characterization of novel cotton promoters with potential application to pest control.Plant cell reports · 2022Article
- Integrated omics approaches for flax improvement under abiotic and biotic stress: Current status and future prospects.Frontiers in plant science · 2022Review
- Genes Associated with the Flax Plant Type (Oil or Fiber) Identified Based on Genome and Transcriptome Sequencing Data.Plants (Basel, Switzerland) · 2021Article
- New Insight into Justicidin B Pathway and Production inInternational journal of molecular sciences · 2021Article
- Article
- Transcriptomes of Different Tissues of Flax (Frontiers in genetics · 2020Article
- The Toolbox for Fiber Flax Breeding: A Pipeline From Gene Expression to Fiber Quality.Frontiers in genetics · 2020Article
- Characterization of repeated DNA sequences in genomes of blue-flowered flax.BMC evolutionary biology · 2019Article
- Flax (Linum usitatissimum L.) response to non-optimal soil acidity and zinc deficiency.BMC plant biology · 2019Article
- The Neuronal Overexpression ofFrontiers in genetics · 2019Article
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Authors and funding
15 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCultivated flax (Linum usitatissimum L.) is widely used for production of textile, food, chemical and pharmaceutical products. However, various stresses decrease flax production. Search for genes, which are involved in stress response, is necessary for breeding of adaptive cultivars. Imbalanced concentration of nutrient elements in soil decrease flax yields and also results in heritable changes in some flax lines. The appearance of Linum Insertion Sequence 1 (LIS-1) is the most studied modification. However, LIS-1 function is still unclear.
resultsHigh-throughput sequencing of transcriptome of flax plants grown under normal (N), phosphate deficient (P), and nutrient excess (NPK) conditions was carried out using Illumina platform. The assembly of transcriptome was performed, and a total of 34924, 33797, and 33698 unique transcripts for N, P, and NPK sequencing libraries were identified, respectively. We have not revealed any LIS-1 derived mRNA in our sequencing data. The analysis of high-throughput sequencing data allowed us to identify genes with potentially differential expression under imbalanced nutrition. For further investigation with qPCR, 15 genes were chosen and their expression levels were evaluated in the extended sampling of 31 flax plants. Significant expression alterations were revealed for genes encoding WRKY and JAZ protein families under P and NPK conditions. Moreover, the alterations of WRKY family genes differed depending on LIS-1 presence in flax plant genome. Besides, we revealed slight and LIS-1 independent mRNA level changes of KRP2 and ING1 genes, which are adjacent to LIS-1, under nutrition stress.
conclusionsDifferentially expressed genes were identified in flax plants, which were grown under phosphate deficiency and excess nutrition, on the basis of high-throughput sequencing and qPCR data. We showed that WRKY and JAS gene families participate in flax response to imbalanced nutrient content in soil. Besides, we have not identified any mRNA, which could be derived from LIS-1, in our transcriptome sequencing data. Expression of LIS-1 flanking genes, ING1 and KRP2, was suggested not to be nutrient stress-induced. Obtained results provide new insights into edaphic stress response in flax and the role of LIS-1 in these process.
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