Evidence map›Paper›PMID 28104718›Full record

ArticlePlant physiology2017

Bioorthogonal Noncanonical Amino Acid Tagging (BONCAT) Enables Time-Resolved Analysis of Protein Synthesis in Native Plant Tissue.

Weslee S Glenn, Shannon E Stone, Samuel H Ho, Michael J Sweredoski, Annie Moradian, Sonja Hess, Julia Bailey-Serres, David A Tirrell

Open access · bronzeAbstract read
In one paragraph

Article in Plant physiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed
3.0field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 66 citations in OpenAlex.

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  3. Global analysis of protein degradation reveals instability of diverse regulators inProceedings of the National Academy of Sciences of the United States of America · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Weslee S GlennDivision of Chemistry and Chemical Engineering (W.S.G., S.E.S., S.H.H., D.A.T.), and Proteome Exploration Laboratory (M.J.S., A.M., S.H.), California Institute of Technology, Pasadena, California 91125; and.ORCID 0000-0001-7733-7291
Shannon E StoneDivision of Chemistry and Chemical Engineering (W.S.G., S.E.S., S.H.H., D.A.T.), and Proteome Exploration Laboratory (M.J.S., A.M., S.H.), California Institute of Technology, Pasadena, California 91125; and.ORCID 0000-0002-6617-3874
Samuel H HoDivision of Chemistry and Chemical Engineering (W.S.G., S.E.S., S.H.H., D.A.T.), and Proteome Exploration Laboratory (M.J.S., A.M., S.H.), California Institute of Technology, Pasadena, California 91125; and.ORCID 0000-0001-7647-0752
Michael J SweredoskiDivision of Chemistry and Chemical Engineering (W.S.G., S.E.S., S.H.H., D.A.T.), and Proteome Exploration Laboratory (M.J.S., A.M., S.H.), California Institute of Technology, Pasadena, California 91125; and.ORCID 0000-0003-0878-3831
Annie MoradianDivision of Chemistry and Chemical Engineering (W.S.G., S.E.S., S.H.H., D.A.T.), and Proteome Exploration Laboratory (M.J.S., A.M., S.H.), California Institute of Technology, Pasadena, California 91125; and.
Sonja HessDivision of Chemistry and Chemical Engineering (W.S.G., S.E.S., S.H.H., D.A.T.), and Proteome Exploration Laboratory (M.J.S., A.M., S.H.), California Institute of Technology, Pasadena, California 91125; and.ORCID 0000-0002-5904-9816
Julia Bailey-SerresDivision of Chemistry and Chemical Engineering (W.S.G., S.E.S., S.H.H., D.A.T.), and Proteome Exploration Laboratory (M.J.S., A.M., S.H.), California Institute of Technology, Pasadena, California 91125; and.ORCID 0000-0002-8568-7125
David A TirrellDivision of Chemistry and Chemical Engineering (W.S.G., S.E.S., S.H.H., D.A.T.), and Proteome Exploration Laboratory (M.J.S., A.M., S.H.), California Institute of Technology, Pasadena, California 91125; and tirrell@caltech.edu.ORCID 0000-0003-3175-4596
University of California, Riverside · USCalifornia Institute of Technology · US

Funding

Purchase of a Liquid Chromatograph-Orbitrap VELOS mass spectrometerS10RR029594 · NCRR · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI HESS, SONJA · 2011 to 2011
$600k
NCRR NIH HHS S10 RR029594
6 · The paper itself

Abstract

Proteomic plasticity undergirds stress responses in plants, and understanding such responses requires accurate measurement of the extent to which proteins levels are adjusted to counter external stimuli. Here, we adapt bioorthogonal noncanonical amino acid tagging (BONCAT) to interrogate protein synthesis in vegetative Arabidopsis (

Indexed as

Protein BiosynthesisAlanineArabidopsisArabidopsis ProteinsAzidesChromatography, LiquidColoring AgentsDroughtsHot TemperatureImmunoblottingLightReproducibility of ResultsSeedlingsSodium ChlorideStaining and LabelingStress, PhysiologicalAlanineArabidopsis ProteinsAzidesazidohomoalanineColoring AgentsSodium Chloride

Identifiers

PMID28104718
PMCPMC5338676
OpenAlexW2573080176

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.