ArticlePLoS genetics2017
Bombyx mori P-element Somatic Inhibitor (BmPSI) Is a Key Auxiliary Factor for Silkworm Male Sex Determination.
Article in PLoS genetics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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41 citing papers in PubMed, 112 citations in OpenAlex.
- The complete sequence of the silkworm W chromosome uncovers its rapid evolution by large-scale duplications/deletions and translocation of W-linked genes.G3 (Bethesda, Md.) · 2026Article
- fmd/fmd2/DHX16-dsx cascade in sex determination of one Hemiptera species, Laodelphax striatellus.Insect science · 2026Article
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- Molecular mechanisms of sex determination in Lepidoptera: current status and perspectives.Insect science · 2026Review
- The Masc-PSI complex directly induces male-type doublesex splicing in silkworms.Communications biology · 2025Article
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- Consistency, distinction, and potential metabolic crosstalk of nitrogen mobilization-related genes in silk production and silk gland biology.Zoological research · 2025Article
- Wingless strain created using binary transgenic CRISPR/Cas9 alleviates concerns about mass rearing of Hermetia illucens.Communications biology · 2024Article
- The T2T Genome of the Domesticated SilkwormInternational journal of molecular sciences · 2024Article
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- High-throughput and genome-scale targeted mutagenesis using CRISPR in a nonmodel multicellular organism,Genome research · 2024Article
- Fzr regulates silk gland growth by promoting endoreplication and protein synthesis in the silkworm.PLoS genetics · 2023Article
- The Prmt5-Vasa module is essential for spermatogenesis in Bombyx mori.PLoS genetics · 2023Article
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- The Evaluation of the Biological Effects of Melanin by Using Silkworm as a Model Animal.Toxins · 2022Article
- Manipulating Insect Sex Determination Pathways for Genetic Pest Management: Opportunities and Challenges.Frontiers in bioengineering and biotechnology · 2022Review
- CRISPR/Cas9-mediatedFrontiers in physiology · 2022Article
- The receptor tyrosine kinase torso regulates ecdysone homeostasis to control developmental timing in Bombyx mori.Insect science · 2021Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Manipulation of sex determination pathways in insects provides the basis for a wide spectrum of strategies to benefit agriculture and public health. Furthermore, insects display a remarkable diversity in the genetic pathways that lead to sex differentiation. The silkworm, Bombyx mori, has been cultivated by humans as a beneficial insect for over two millennia, and more recently as a model system for studying lepidopteran genetics and development. Previous studies have identified the B. mori Fem piRNA as the primary female determining factor and BmMasc as its downstream target, while the genetic scenario for male sex determination was still unclear. In the current study, we exploite the transgenic CRISPR/Cas9 system to generate a comprehensive set of knockout mutations in genes BmSxl, Bmtra2, BmImp, BmImpM, BmPSI and BmMasc, to investigate their roles in silkworm sex determination. Absence of Bmtra2 results in the complete depletion of Bmdsx transcripts, which is the conserved downstream factor in the sex determination pathway, and induces embryonic lethality. Loss of BmImp or BmImpM function does not affect the sexual differentiation. Mutations in BmPSI and BmMasc genes affect the splicing of Bmdsx and the female reproductive apparatus appeared in the male external genital. Intriguingly, we identify that BmPSI regulates expression of BmMasc, BmImpM and Bmdsx, supporting the conclusion that it acts as a key auxiliary factor in silkworm male sex determination.
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