ArticleLab on a chip2017
Brillouin flow cytometry for label-free mechanical phenotyping of the nucleus.
Article in Lab on a chip, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 75 citations in OpenAlex.
- Nuclear mechanotransduction: tools for mechanical perturbation and chromatin characterization.Nucleus (Austin, Tex.) · 2026Review
- Multiscale Architecture and Mechanics of the Cell Nucleus: Implications for Disease, Bioengineering and Nanomedicine.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Stabilized real-time Brillouin microscopy reveals fractal organization of protein condensates in living cells.Nature communications · 2026Article
- Polarization increases nuclear stiffness in macrophages despite reduction in lamin A/C levels.npj biological physics and mechanics · 2026Article
- Stimulated Brillouin scattering flow cytometry.Biomedical optics express · 2024Article
- Exploiting Matrix Stiffness to Overcome Drug Resistance.ACS biomaterials science & engineering · 2024Review
- Article
- Progressive alteration of murine bladder elasticity in actinic cystitis detected by Brillouin microscopy.Scientific reports · 2024Article
- Brillouin microscopy.Nature reviews. Methods primers · 2024Article
- A Novel Role of Connective Tissue Growth Factor in the Regulation of the Epithelial Phenotype.Cancers · 2023Article
- Rapid biomechanical imaging at low irradiation level via dual line-scanning Brillouin microscopy.Nature methods · 2023Article
- Comprehensive single-shot biophysical cytometry using simultaneous quantitative phase imaging and Brillouin spectroscopy.Scientific reports · 2022Article
- Multimodal microscale mechanical mapping of cancer cells in complex microenvironments.Biophysical journal · 2022Article
- Changes in intra-nuclear mechanics in response to DNA damaging agents revealed by time-domain Brillouin micro-spectroscopy.Photoacoustics · 2022Article
- Correlation of biomechanics and cancer cell phenotype by combined Brillouin and Raman spectroscopy of U87-MG glioblastoma cells.Journal of the Royal Society, Interface · 2022Article
- Amoeboid-like migration ensures correct horizontal cell layer formation in the developing vertebrate retina.eLife · 2022Article
- Localization-assisted stimulated Brillouin scattering spectroscopy.APL photonics · 2022Article
- Non-contact elastography methods in mechanobiology: a point of view.European biophysics journal : EBJ · 2022Review
- Brillouin Spectroscopy: From Biomedical Research to New Generation Pathology Diagnosis.International journal of molecular sciences · 2021Review
- Tumor cell nuclei soften during transendothelial migration.Journal of biomechanics · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 3 countries.
Funding
Abstract
The mechanical properties of the nucleus are closely related to many cellular functions; thus, measuring nuclear mechanical properties is crucial to our understanding of cell biomechanics and could lead to intrinsic biophysical contrast mechanisms to classify cells. Although many technologies have been developed to characterize cell stiffness, they generally require contact with the cell and thus cannot provide direct information on nuclear mechanical properties. In this work, we developed a flow cytometry technique based on an all-optical measurement to measure nuclear mechanical properties by integrating Brillouin spectroscopy with microfluidics. Brillouin spectroscopy probes the mechanical properties of material via light scattering, so it is inherently label-free, non-contact, and non-invasive. Using a measuring beam spot of submicron size, we can measure several regions within each cell as they flow, which enables us to classify cell populations based on their nuclear mechanical signatures at a throughput of ∼200 cells per hour. We show that Brillouin cytometry has sufficient sensitivity to detect physiologically-relevant changes in nuclear stiffness by probing the effect of drug-induced chromatin decondensation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.