ArticleNature communications2017
The impact of microRNAs on transcriptional heterogeneity and gene co-expression across single embryonic stem cells.
Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed.
- Landscape of microRNA and target expression variation and covariation in single mouse embryonic stem cells.Genome research · 2026Article
- Feedforward miR-181d degradation modulates population variance of methyl-guanine methyl transferase and temozolomide resistance.Cell reports · 2025Article
- Tanshinone IIA inhibits neuronal ferroptosis and relieves cerebral ischemia‒reperfusion injury by regulating miR-449a/ACSL4.Metabolic brain disease · 2025Article
- MIRELLA: a mathematical model explains the effect of microRNA-mediated synthetic genes regulation on intracellular resource allocation.Nucleic acids research · 2023Article
- Exosome-mediated inhibition of microRNA-449a promotes the amplification of mouse retinal progenitor cells and enhances their transplantation in retinal degeneration mouse models.Molecular therapy. Nucleic acids · 2023Article
- G1/S restriction point coordinates phasic gene expression and cell differentiation.Nature communications · 2022Article
- A Combined mRNA- and miRNA-Sequencing Approach Reveals miRNAs as Potential Regulators of the Small Intestinal Transcriptome in Celiac Disease.International journal of molecular sciences · 2021Article
- Cancer of unknown primary stem-like cells model multi-organ metastasis and unveil liability to MEK inhibition.Nature communications · 2021Article
- The multifaceted roles of microRNAs in differentiation.Current opinion in cell biology · 2020Review
- Characterization and mitigation of gene expression burden in mammalian cells.Nature communications · 2020Article
- The Key Role of MicroRNAs in Self-Renewal and Differentiation of Embryonic Stem Cells.International journal of molecular sciences · 2020Review
- High-throughput intracellular biopsy of microRNAs for dissecting the temporal dynamics of cellular heterogeneity.Science advances · 2020Article
- The translational landscape of ground state pluripotency.Nature communications · 2020Article
- Regulation of stomatal development by stomatal lineage miRNAs.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Let-7 as biomarker, prognostic indicator, and therapy for precision medicine in cancer.Clinical and translational medicine · 2019Review
- Up-regulation of miR-155 contributes to TNF-mediated hepatocyte apoptosis in acute liver failure.The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology · 2019Article
- Dgcr8 knockout approaches to understand microRNA functions in vitro and in vivo.Cellular and molecular life sciences : CMLS · 2019Review
- A Tool for Visualization and Analysis of Single-Cell RNA-Seq Data Based on Text Mining.Frontiers in genetics · 2019Article
- Article
- Stochastic Modeling of Gene Regulation by Noncoding Small RNAs in the Strong Interaction Limit.Biophysical journal · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
MicroRNAs act posttranscriptionally to suppress multiple target genes within a cell population. To what extent this multi-target suppression occurs in individual cells and how it impacts transcriptional heterogeneity and gene co-expression remains unknown. Here we used single-cell sequencing combined with introduction of individual microRNAs. miR-294 and let-7c were introduced into otherwise microRNA-deficient Dgcr8 knockout mouse embryonic stem cells. Both microRNAs induce suppression and correlated expression of their respective gene targets. The two microRNAs had opposing effects on transcriptional heterogeneity within the cell population, with let-7c increasing and miR-294 decreasing the heterogeneity between cells. Furthermore, let-7c promotes, whereas miR-294 suppresses, the phasing of cell cycle genes. These results show at the individual cell level how a microRNA simultaneously has impacts on its many targets and how that in turn can influence a population of cells. The findings have important implications in the understanding of how microRNAs influence the co-expression of genes and pathways, and thus ultimately cell fate.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.